Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development
Article first published online: 13 MAY 2009
Copyright © 2009 Wiley-Liss, Inc.
Special Issue: Special Focus on Xenopus
Volume 238, Issue 6, pages 1574–1581, June 2009
How to Cite
Park, J.-S., Kim, H.-S., Kim, J.-D., Seo, J., Chung, K.-S., Lee, H.-S., Huh, T.-L., Jo, I. and Kim, Y.-O. (2009), Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development. Dev. Dyn., 238: 1574–1581. doi: 10.1002/dvdy.21964
- Issue published online: 13 MAY 2009
- Article first published online: 13 MAY 2009
- Manuscript Accepted: 20 MAR 2009
- Korea National Institute of Health. Grant Number: 4845-300-210
We investigated chamber-specific gene expression by isolating a 2.2-kb polymerase chain reaction product containing the 5′-flanking region of the zebrafish ventricular myosin heavy-chain gene (vmhc). Promoter analysis revealed that the fragment, consisting of nucleotides from −301 to +26, is sufficient for vmhc promoter activity. Among several putative cis-acting elements in the region, a GATA-binding site was identified to be crucial for the activity of the promoter, as evidenced by the complete abolishment of promoter activity by a single nucleotide substitution of GATA-binding site (−287, CT). Knockdown of GATA-binding proteins 4 and 6 (GATA4 and -6) by their antisense morpholino oligonucleotides resulted in reduced green fluorescent protein (GFP) reporter gene and endogenous vmhc expression. These findings suggest that GATA4 and -6 play a key role in the regulation of vmhc expression in the ventricle. In addition, the vmhc promoter and the transgenic zebrafish (vmhc:gfp) are useful tools to study the formation and function of the ventricle. Developmental Dynamics 238:1574–1581, 2009. © 2009 Wiley-Liss, Inc.