SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
DVDY_21970_sm_SupMovieS1.mov5768KSupp. Movie S1. Spinal cord precursor cleavage is vertical to the central canal. Excerpt from time-lapse sequence of a Tg(h2afv:egfp) embryo. Images are from a dorsal view and focused on the trunk region of the spinal cord with anterior to the right. Sequence shown begins at 27 hours postfertilization (hpf) and ends at approximately 34 hpf. The central canal is represented by the enhanced green fluorescent protein–negative (EGFP−) space in the center of the movie. All cells that divide close to the central canal have near-vertical cleavage planes. Images were collected every 4 min and the movie runs 3 frames/sec.
DVDY_21970_sm_SupMovieS2.mov4737KSupp. Movie S2. Spinal cord precursor divisions are oblique to the central canal in has mutant embryos. Excerpt from a time-lapse sequence of a has−/−;Tg(olig2:egfp embryo. Images are from a dorsal view and focused on the trunk region of the spinal cord with anterior to the right. Sequence shown start at 27 hours postfertilization (hpf) and ends at approximately 35 hpf. The central canal is represented by the enhanced green fluorescent protein–negative (EGFP−) space in the center of the movie. At the beginning of the movie most cleavages of cells that divide near the central canal are near-vertical. As the movie progresses, many cleavage planes become oblique and the central canal is replaced by cells. Images were collected every 4 min and the movie runs 3 frames per sec.
DVDY_21970_sm_SupMovieS3.mov5032KSupp. Movie S3. olig2+ oligodendrocyte progenitor cell (OPC) migration from ventral spinal cord. Excerpt from a time-lapse sequence of a Tg(olig2:egfp) embryo. The images are from a lateral view focused on the trunk region of the spinal cord. Dorsal is up and anterior is to the right. Sequence begins at 50 hours postfertilization (hpf) and ends at approximately 66 hpf. enhanced green fluorescent protein–positive (EGFP+) moving upward are OPCs migrating from the EGFP+ pMN domain into dorsal spinal cord. Images were collected every 15 min and the movie runs 3 frames per sec.
DVDY_21970_sm_SupMovieS4.mov4439KSupp. Movie S4. Excess olig2+ oligodendrocyte progenitor cells (OPCs) are produced in the absence of aPKCλ function. Excerpt from a time-lapse sequence of a has−/−;Tg(olig2:egfp) embryo. The images are from a lateral view focused on the trunk region of the spinal cord. Dorsal is up and anterior is to the right. Sequence shown begins at 50 hours postfertilization (hpf) and ends at approximately 66 hpf. More OPCs migrate from ventral pMN domain into dorsal spinal cord than in wild-type. Images were collected every 15 min and the movie runs 3 frames per sec.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.