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Additional Supporting Information may be found in the online version of this article.

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DVDY_21973_sm_SupFigS1.tif1633KSupp. Fig. S1. Clusters of genes expressed in the developing ciliary body. Hierarchical clustering of 44 microarrays including developing RGCs, ACs, PRs, cycling RPCs, and the two putative ciliary body cells. A: Genes that clustered with Cyclin D2 with a correlation coefficient of >0.7 are shown. B: Genes that clustered with Foxp2 with a correlation coefficient of >0.7 are shown.
DVDY_21973_sm_SupFigS2.tif6299KSupp. Fig. S2. ISH analysis of candidate genes for the peripheral mouse and chick eye. ISH on retinal cryosections was performed at three stages of mouse development: E12.5 (A, D, G, J), E16.5 (B, E, H, K), and P0 (C, F, I, L) and two stages of chick development: E6 (M) and E8 (N). The following probes were used: AI848240 (Zic1) (A–C), BE988982 (Zic2) (D–F), AI842763 (Rhbdf1) (G–I), AW045815 (Irs4) (J–L), and vinculin (M, N).
DVDY_21973_sm_SupFigS3.tif4331KSupp. Fig. S3. Further characterization of H19 expression. Close-ups of in situ hybridizations using an H19 riboprobe (BE949664) and E16.5 mouse retinas (A, B). The ONBL is denoted by a red bar. P0 retinas were explanted, labeled with [3H]-thymidine for 1 hr, dissociated, and probed for H19 alone (C, D) or for H19 (red) and Fgf15 (green) (E, F). Images are shown with (C, E) and without (D, F) the development of the [3H]-thymidine. The arrows in E indicate cells that are positive for both H19 and Fgf15.
DVDY_21973_sm_SupFigS4.tif3107KSupp. Fig. S4. A comparison of ciliary body/iris marker genes from this study and others. A: A Treeview-generated heat-map showing the expression of 6 previously identified ciliary body marker genes (Thut et al., 2001) in E12 cell A6, P0 cell E8, along with the single RGCs, ACs, and PRs. ISH for Tgfb1i4 was performed on retinal cryosections at E12.5 (B), E14.5 (C), E16.5 (D), and P0 (E). Arrows indicate the location of the INBL staining. Scale bars are shown.
DVDY_21973_sm_SupFigS5.tif1226KSupp. Fig. S5. A comparison of ciliary body/iris marker genes from this study and others. A: A Treeview-generated heat-map showing the expression of a set of previously identified adult human ciliary body marker genes (Diehn et al., 2005) in E12 cell A6, P0 cell E8, along with the single RGCs, ACs, and PRs. B: A Treeview-generated heat-map showing the expression of a second set of previously identified adult human ciliary body marker genes (Escribano and Coca-Prados, 2002) in E12 cell A6, P0 cell E8, along with the single RGCs, ACs, and PRs.
DVDY_21973_sm_SupTableS1.xls25KSupp. Table 1. Summary of gene expression in the mouse and chicken embryos using ISH and the probes used in this study. For each gene, the corresponding cDNA used to generate the ISH riboprobe in both the chick and mouse is listed. A summary of the expression pattern observed at each stage is included. ONBL, Outer neuroblastic layer/ventricular zone; INBL, inner neuroblastic layer; RPE, retinal pigment epithelium; ND, not detectable; NT, not tested; NA, no probes available.

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