In vitro organogenesis from undifferentiated cells in Xenopus

Authors

  • Makoto Asashima,

    Corresponding author
    1. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, Japan
    2. Organ Development Research Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, Tsukuba, Ibaraki, Japan
    • Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan
    Search for more papers by this author
  • Yuzuru Ito,

    1. Organ Development Research Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, Tsukuba, Ibaraki, Japan
    Search for more papers by this author
  • Techuan Chan,

    1. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, Japan
    Search for more papers by this author
  • Tatsuo Michiue,

    1. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, Japan
    Search for more papers by this author
  • Mio Nakanishi,

    1. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, Japan
    Search for more papers by this author
  • Kan Suzuki,

    1. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, Japan
    Search for more papers by this author
  • Keisuke Hitachi,

    1. Institute for Comprehensive Medical Sciences, Fujita Health University, Toyoake, Aichi, Japan
    Search for more papers by this author
  • Koji Okabayashi,

    1. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, Japan
    Search for more papers by this author
  • Akiko Kondow,

    1. Institute for Comprehensive Medical Sciences, Fujita Health University, Toyoake, Aichi, Japan
    Search for more papers by this author
  • Takashi Ariizumi

    1. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, Japan
    Search for more papers by this author

Abstract

Amphibians have been used for over a century as experimental animals. In the field of developmental biology in particular, much knowledge has been accumulated from studies on amphibians, mainly because they are easy to observe and handle. Xenopus laevis is one of the most intensely investigated amphibians in developmental biology at the molecular level. Thus, Xenopus is highly suitable for studies on the mechanisms of organ differentiation from not only a single fertilized egg, as in normal development, but also from undifferentiated cells, as in the case of in vitro organogenesis. Based on the established in vitro organogenesis methods, we have identified many genes that are indispensable for normal development in various organs. These experimental systems are useful for investigations of embryonic development and for advancing regenerative medicine. Developmental Dynamics 238:1309–1320, 2009. © 2009 Wiley-Liss, Inc.

Ancillary