This article was accepted for inclusion in Developmental Dynamics238#6 – Special Focus on Xenopus.
Special Issue Research Article
Role of p21-activated kinase in cell polarity and directional mesendoderm migration in the Xenopus gastrula†
Version of Record online: 4 JUN 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 7, pages 1709–1726, July 2009
How to Cite
Nagel, M., Luu, O., Bisson, N., Macanovic, B., Moss, T. and Winklbauer, R. (2009), Role of p21-activated kinase in cell polarity and directional mesendoderm migration in the Xenopus gastrula. Dev. Dyn., 238: 1709–1726. doi: 10.1002/dvdy.21985
- Issue online: 10 JUN 2009
- Version of Record online: 4 JUN 2009
- Manuscript Accepted: 17 APR 2009
- Canadian Institutes of Health Research. Grant Number: MOP-53075
- National Cancer Institute of Canada
- p21-activated kinase;
The p21 activated kinases (Paks) are prominently involved in the regulation of cell motility. Using a kinase-dead mutant of xPak1, we show that during Xenopus gastrulation, the kinase activity of Pak1 is required upstream of Cdc42 for the establishment of cell polarity in the migrating mesendoderm. Overactivation of Pak1 function by the expression of constitutively active xPak1 compromises the maintenance of cell polarity, by indirectly inhibiting RhoA function. Inhibition of cell polarization does not affect the migration of single mesendoderm cells. However, Pak1 inhibition interferes with the guidance of mesendoderm migration by directional cues residing in the extracellular matrix of the blastocoel roof, and with mesendoderm translocation in the embryo. Developmental Dynamics 238:1709–1726, 2009. © 2009 Wiley-Liss, Inc.