Drs. Russek-Blum and Nabel-Rosen contributed equally to this work.
Patterns & Phenotypes
High resolution fate map of the zebrafish diencephalon
Article first published online: 4 JUN 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 7, pages 1827–1835, July 2009
How to Cite
Russek-Blum, N., Nabel-Rosen, H. and Levkowitz, G. (2009), High resolution fate map of the zebrafish diencephalon. Dev. Dyn., 238: 1827–1835. doi: 10.1002/dvdy.21987
- Issue published online: 10 JUN 2009
- Article first published online: 4 JUN 2009
- Manuscript Accepted: 14 APR 2009
- Dekker Foundation
- German-Israeli Foundation. Grant Number: 183/2007
- Israel Science Foundation. Grant Number: 928/08
- Minna James Heineman Foundation
- National Institute for Psychobiology in Israel
- neural progenitors;
- forebrain development;
- dopaminergic neurons;
- two photon microscopy;
- neural patterning
The diencephalon acts as an interactive site between the sensory, central, and endocrine systems and is one of the most elaborate structures in the vertebrate brain. To better understand the embryonic development and morphogenesis of the diencephalon, we developed an improved photoactivation (uncaging)-based lineage tracing strategy. To determine the exact position of a given diencephalic progenitor domain, we used a transgenic line driving green fluorescent protein (GFP) in cells expressing the proneural protein, Neurogenin1 (Neurog1), which was used as a visible neural plate landmark. This approach facilitated precise labeling of defined groups of cells in the prospective diencephalon of the zebrafish neural plate. In this manner, we labeled multiple overlapping areas of the diencephalon, thereby ensuring both accuracy and reproducibility of our lineage tracing regardless of the dynamic changes of the developing neural plate. We present a fate map of the zebrafish diencephalon at a higher spatial resolution than previously described. Developmental Dynamics 238:1827–1835, 2009. © 2009 Wiley-Liss, Inc.