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FilenameFormatSizeDescription
DVDY_21990_sm_SuppFigS1.eps1856KFigure S1. Zebrafish Angiotensin Receptor 2 sequence and homology analysis. (A) Zebrafish Agtr2 contains 7-transmembrane domains as revealed by NCBI Conserved Domain Database (CDD) search (v2.14)(Marchler-Bauer et al., 2007). (B) Homology tree of agtr1 and agtr2 subfamilies displaying homologs from human, mouse, frog Xenopus laevis, chick and zebrafish. (C) Alignment of zebrafish Agtr2 protein sequence with its human and mouse Agtr2 homologs as well as with more distant human Agtr1. Identical amino acids are shaded in dark grey while similar amino acids are shaded in medium grey.
DVDY_21990_sm_SuppFigS2.eps2840KFigure S2. Zebrafish Mannose Receptor C1 sequence and homology analysis. (A) N-terminal Ricin-type beta-trefoil, fibronectin type II and C-type lectin-like domains are predicted by CDD search. (B) Homology tree of Mrc1 and Mrc2 subfamilies from mouse, human, chick and zebrafish. Two Mrc1 homologs are predicted within zebrafish genome. (C) Alignment of protein sequences from the zebrafish, human and mouse Mrc1 homologs. Identical amino acids are shaded in dark grey while similar amino acids are shaded in medium grey. The first 333 a.a. of zebrafish Mrc1a sequence are based on a prediction from genomic sequence while the rest have been confirmed by cDNA sequencing (Genbank Accession Number FJ205703).
DVDY_21990_sm_SuppFigS3.eps1904KFigure S3. Zebrafish Src homology domain 2 containing E (She) sequence and homology analysis. (A) She contains C-terminal SH2 domain as predicted by CDD search. (B) Homology tree of she, shd, shf and shb subfamilies from zebrafish, human, mouse and chick. (C) Alignment of predicted zebrafish, human and mouse She protein sequences. Identical amino acids are shaded in grey.
DVDY_21990_sm_SuppFigS4.eps1988KFigure S4. Zebrafish Esam sequence and homology analysis. (A) Zebrafish Esam contains two immunoglobulin domains, including a V-set domain as predicted by CDD search. Predicted transmembrane and cytoplasmic domains are not shown. (B) Homology tree of related Esam and Immunoglobulin superfamily, member 11 (IGSF11) subfamilies from zebrafish, chick, mouse and humans. (C) Alignment of zebrafish ESAMa and ESAMb protein sequences with its closest human and mouse homologs. Identical amino acids are shaded in dark grey, while similar amino acids are shaded in medium grey. ESAMa cDNA sequence has been submitted to GenBank with accession number FJ205704.
DVDY_21990_sm_SuppFigS5.eps2107KFigure S5. Zebrafish Yrk sequence and homology analysis. (A) Zebrafish Yrk contains Src homology 2 and protein tyrosine kinase catalytic domains based on CDD homology analysis. (B) Homology tree of Yrk, Fyn, Yes, Src and Fgr protein kinases from human, mouse, chick, frog Xenopus laevis and zebrafish. (C) Alignment of two zebrafish Yrk/Fyn homologs with their closest homologs from mouse and human. Zebrafish yrk cDNA sequence has been submitted to GenBank with accession number FJ205701.
DVDY_21990_sm_SuppFigS6.tif53KFigure S6. Confirmation of fog2b downregulation in etsrp morphants by RT-PCR analysis. Total RNA from control and morphant embryos at the 16-somite stage was purified and used for cDNA synthesis. EF1? expression was used as a control reference. Primer sequences are listed in Table S1.
DVDY_21990_sm_SuppFigS7.eps4515KFigure S7. Zebrafish Stabilin 2 sequence and homology analysis. (A) Stab2 is predicted to contain 7 fasciclin-like adhesion domains, an X-link domain and putative hyaluronan binding sites based on CDD analysis. In addition, Stab2 proteins contain 18-20 EGF domains and a transmembrane domain (not shown). (B) Homology tree of Stab1 and Stab2 subfamilies from zebrafish, chick, mouse and humans. (C) Alignment of mouse, human and zebrafish Stab2 sequences. Zebrafish stab2 cDNA sequence has been submitted to GenBank with an Accession Number FJ945321.
DVDY_21990_sm_SuppTableS1.doc39KTable S1. Primer sequences used for the confirmation of the microarray results by the real-time RT-PCR.
DVDY_21990_sm_SuppTableS2.doc43KTable S2. Vector information and probe synthesis methods.

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