Special Issue Research Article
Early lens development in the zebrafish: A three-dimensional time-lapse analysis
Article first published online: 4 JUN 2009
Copyright © 2009 Wiley-Liss, Inc.
Special Issue: Special Focus on Visual Systems
Volume 238, Issue 9, pages 2254–2265, September 2009
How to Cite
Greiling, T. M.S. and Clark, J. I. (2009), Early lens development in the zebrafish: A three-dimensional time-lapse analysis. Dev. Dyn., 238: 2254–2265. doi: 10.1002/dvdy.21997
- Issue published online: 13 AUG 2009
- Article first published online: 4 JUN 2009
- Manuscript Accepted: 29 APR 2009
- NIH. Grant Numbers: EY04542, EY07031
- lens mass;
In vivo, high-resolution, time-lapse imaging characterized lens development in the zebrafish from 16 to 96 hr postfertilization (hpf). In zebrafish, the lens placode appeared in the head ectoderm, similar to mammals. Delamination of the surface ectoderm resulted in the formation of the lens mass, which progressed to a solid sphere of cells separating from the developing cornea at approximately 24 hpf. A lens vesicle was not observed and apoptosis was not a major factor in separation of the lens from the future cornea. Differentiation of primary fibers began in the lens mass followed by formation of the anterior epithelium after delamination was complete. Secondary fibers differentiated from elongating epithelial cells near the posterior pole. Quantification characterized three stages of lens growth. The study confirmed the advantages of live-cell imaging for three-dimensional quantitative structural characterization of the mechanism(s) responsible for cell differentiation in formation of a transparent, symmetric, and refractile lens. Developmental Dynamics 238:2254–2265, 2009. © 2009 Wiley-Liss, Inc.