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DVDY21997SuppMovieS1.mov2854KSupp. Movie S1. At 16 hours postfertilization (hpf), lens placode. 00.00–04.08 sec: Movie scrolls dorsal/ventrally through axial sections of the eye. A single layer of surface ectoderm formed the columnar cells of the lens placode, overlying and closely apposed to the developing retina. Laterally, the surface ectoderm remains cuboidal. The width of each section is 143.9 μm. 04.09–06.08 sec: In rotation, the three-dimensional (3D) projection of the surface ectoderm (gray) includes colored, space-filled columnar placode cells. The circular organization of the placode is readily visualized. 06.09–08.16 sec: Space-filled cells of the placode demonstrated uniformity of cell size and shape.
DVDY21997SuppMovieS2.mov3319KSupp. Movie S2. At 18 hours postfertilization (hpf), lens mass. 00.00–03.23 sec: Movie scrolls dorsal/ventrally through axial sections of the eye. At the start of the formation of the lens mass, the placode is multilayered and organized into elongated columnar cells laterally and more ovoid cells at the center surface. The width of each section is 127.0 μm. 04.00–05.23 sec: The lens mass and surface ectoderm are shown as a rotating three-dimensional (3D) projection with the anterior/central ovoid cells colored. Small, round cells (blue) appear to be apoptotic above the anterior surface of the lens mass. Note the circular shape of the lens mass when viewed from the posterior surface at 05.11 sec. 06.00–07.00 sec: The lens mass alone is shown as a rotating 3D projection.
DVDY21997SuppMovieS3.mov3921KSupp. Movie S3. At 20 hours postfertilization (hpf), lens mass and beginning of delamination. 00.00–02.06 sec: Movie scrolls dorsal/ventrally through axial sections of the eye. The lens mass thickens to bulge toward the retina. Elongated cells form an organized layer along the posterior and lateral borders of the lens mass and surround a central stalk of ovoid cells. Small, apoptotic-appearing cells are visible above the anterior surface. The width of each section is 107.0 μm. 03.08–04.09 sec: The central stalk of cells (space-filled) are oriented more randomly than the surrounding elongated cells. 04.10–06.00 sec: The lens mass and developing cornea area shown as a rotating three-dimensional (3D) projection with the central stalk colored. Note the lens mass begins to form a spherical shape.
DVDY21997SuppMovieS4.mov3562KSupp. Movie S4. At 22 hours postfertilization (hpf), delaminating lens mass. 00.01–03.23 sec: Scrolling dorsal/ventrally through axial sections of the eye. The connection between the lens stalk and cornea narrows to approximately two to three cells in diameter. Elongated fiber-like cells form an organized layer at the posterior and lateral borders, radiating out from a central core of ovoid cells. Each section is 101.4 μm wide. 04.06–05.22 sec: In rotation, the three-dimensional (3D) projection of the lens and cornea include a central core of developing primary fiber cells in color. 05.23–07.00 sec: Selected fiber-like cells space-filled (green) to show radial organization.
DVDY21997SuppMovieS5.mov3965KSupp. Movie S5. At 23 hours postfertilization (hpf), detachment from the surface ectoderm. 00.01–04.11 sec: Scrolling dorsal/ventrally through axial sections of the eye. The stalk between lens and cornea narrows to the diameter of a single cell. As at 22 hpf, elongated fiber-like cells radiate out from a central core of oval cells in the developing lens nucleus. Each section is 101.4μm wide. 04.19–06.17 sec: In rotation, the three-dimensional (3D) projection includes the lens and cornea with central cells in color. 06.23–08.16 sec: Cells of the lens stalk and cornea are space-filled. Only the red cell and purple cell connect the lens to the cornea.
DVDY21997SuppMovieS6.mov3433KSupp. Movie S6. At 24 hours postfertilization (hpf), after detachment. 00.01–03.23 sec: Scrolling dorsal/ventrally through axial sections of the eye. A narrow space is visible between the developing lens and cornea which have separated. Developing primary fibers curve around the central core of the lens nucleus. Note interdigitations visible between lateral membranes of fibers cells at 01.03 sec. Section width 101.4 μm. 04.04–07.23: Rotating three-dimensional (3D) projection view of separated lens and cornea with central core cells colored.
DVDY21997SuppMovieS7A.mov2197KSupp. Movie S7A. At 24 hours postfertilization (hpf), lens during final stages of detachment. Movie scrolls dorsal/ventrally through axial sections of the eye. The connection between the lens and cornea can be seen at 03.18 sec. As in the previous movie, primary fiber cells wrap around a central core. Anteriorly, cells begin to organize into a single layer of epithelium. Cells deep to this appear disorganized and are less easily distinguished. Section width 127.4 μm.
DVDY21997SuppMovieS7A1.mov3535KSupp. Movie S7A1. At 26 hours postfertilization (hpf). Movie scrolls medial/laterally through equatorial sections of the eye. A section through the middle of the lens at 00.01 sec shows rounded cells forming the central core surrounded by layers of flattened fiber cells. A layer of anterior epithelium is forming over the anterior hemisphere, visible from 02.04–04.15 sec. A section through the anterior epithelium at 01.15 sec lacks the interdigitations visible between lateral cell membrane of the posterior fibers visible at 05.10 sec. Section width 127.4 μm.
DVDY21997SuppMovieS7B.mov3797KSupp. Movie S7B. At 28 hours postfertilization (hpf). Movie scrolls dorsal/ventrally through axial sections of the eye. Several layers of primary fibers have been added, wrapping around the central core. An organized layer of anterior epithelium is visible and extends past the lens equator where epithelial cells take on an elongated appearance as if secondary fiber formation had begun. Only a few undifferentiated cells remain deep to the anterior epithelium. Section width 127.4 μm.
DVDY21997SuppMovieS7B1.mov3731KSupp. Movie S7B1. At 29 hours postfertilization (hpf). Movie scrolls medial/laterally through equatorial sections of the eye at a similar stage to that in 7B. Section width 127.4 μm.
DVDY21997SuppMovieS7C.mov3532KSupp. Movie S7C. At 36 hours postfertilization (hpf). Movie scrolls dorsal/ventrally through axial sections of the eye. The anterior epithelium is cuboidal in shape and in direct contact with underlying fiber cells. The anterior epithelium appears to have a clear transition zone for the formation of secondary fiber cells posterior to the equator. The membranes between the large round cells in the lens nucleus have taken on a jagged appearance. Newly added fiber cells are more compact than at 28 hpf. Section width 127.4 μm.
DVDY21997SuppMovieS7C1.mov2540KSupp. Movie S7C1. At 37 hours postfertilization (hpf). Movie scrolls medial/laterally through equatorial sections of the eye at a similar stage to that in 7C. Section width 127.4μm.
DVDY21997SuppMovieS8A.mov3962KSupp. Movie S8A. At 48 hours postfertilization (hpf). Movie scrolls dorsal/ventrally through axial sections of the eye. The flat anterior epithelium with low expression of the cyan fluorescent protein fusion protein is dimmer than at 36 hpf. Fiber cells continue to narrow, and it can be difficult to see cytoplasm in cortical fibers. The developing posterior suture is visible at 03.11 sec (red arrow). The shape of the lens is longer in the anterior/posterior direction than the equatorial width. Section width 127.4 μm.
DVDY21997SuppMovieS8A1.mov2376KSupp. Movie S8A1. At 49 hours postfertilization (hpf). Movie scrolls medial/laterally through equatorial sections of the eye at a similar stage to that in 8A. Section width 127.4 μm.
DVDY21997SuppMovieS8B.mov2774KSupp. Movie S8B. At 72 hours postfertilization (hpf). Movie scrolls dorsal/ventrally through axial sections of the eye. Individual cells in the lens nucleus can no longer be distinguished. The anterior and posterior sutures are visible at 03.11 sec (red arrows). Section width 127.4 μm.
DVDY21997SuppMovieS8B1.mov3589KSupp. Movie S8B1. At 73 hours postfertilization (hpf). Movie scrolls medial/laterally through equatorial sections of the eye at a similar stage to that in 8B. The anterior suture is visible at 01.03 sec, and the posterior suture is visible at 04.12 sec. Section width 127.4 μm.
DVDY21997SuppMovieS8C.mov3876KSupp. Movie S8C. At 96 hours postfertilization (hpf). Movie scrolls dorsal/ventrally through axial sections of the eye. A larger region of the lens nucleus contains cells in which the membrane outline is not visible. The anterior epithelium is flat with dimmer cyan fluorescent protein expression than in fiber cells. The sutures are visible at 03.17 sec (red arrows). Section width 127.4 μm.
DVDY21997SuppMovieS8C1.mov3004KSupp. Movie S8C1. At 97 hours postfertilization (hpf). Movie scrolls medial/laterally through equatorial sections of the eye at a similar stage to that in 8C. The anterior suture is visible at 01.06 sec, and the posterior suture is visible at 04.22 sec. Section width 127.4μm.

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