T.M. Smith and X. Wang contributed equally to this work.
Hoxa2 plays a direct role in murine palate development
Article first published online: 3 AUG 2009
Copyright © 2009 Wiley-Liss, Inc.
Special Issue: Special Focus on Visual Systems
Volume 238, Issue 9, pages 2364–2373, September 2009
How to Cite
Smith, T. M., Wang, X., Zhang, W., Kulyk, W. and Nazarali, A. J. (2009), Hoxa2 plays a direct role in murine palate development. Dev. Dyn., 238: 2364–2373. doi: 10.1002/dvdy.22040
- Issue published online: 13 AUG 2009
- Article first published online: 3 AUG 2009
- Manuscript Accepted: 14 JUN 2009
- Natural Sciences and Engineering Research Council of Canada
- secondary palate;
- Hox genes;
- cleft palate;
- downstream targets;
The cleft palate exhibited by Hoxa2 null murine embryos has been described as being secondary to abnormalities of tongue musculature, and Hoxa2 was presumed to not play a direct role in palate development. However, we detected Hoxa2 expression in the developing palate at both the mRNA and protein levels between embryonic day (E) 12.5 and E15.5. Organ cultures of Hoxa2−/− palates maintained in the absence of the tongue showed decreased fusion rates than either Hoxa2+/− or Hoxa2+/+ palate cultures. Knocking down Hoxa2 expression with antisense retroviral constructs resulted in decreased fusion rates than corresponding controls. An overall increase in cell proliferation was observed in Hoxa2 null palates providing a potential mechanism by which Hoxa2 may be affecting palate development. Hoxa2 also repressed the expression of its downstream targets Msx1, Bmp4, Barx1, and Ptx1 within the palate. These results demonstrate the cleft palate phenotype of Hoxa2 null embryos is not solely due to abnormal tongue musculature, and indicate a direct role of Hoxa2 in regulating murine palatogenesis. Developmental Dynamics 238:2364–2373, 2009. © 2009 Wiley-Liss, Inc.