Patterns & Phenotypes
Platelet-derived growth factor is involved in the differentiation of second heart field-derived cardiac structures in chicken embryos
Article first published online: 24 AUG 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 238, Issue 10, pages 2658–2669, October 2009
How to Cite
Bax, N. A.M., Lie-Venema, H., Vicente-Steijn, R., Bleyl, S. B., Van Den Akker, N. M.S., Maas, S., Poelmann, R. E. and Gittenberger-de Groot, A. C. (2009), Platelet-derived growth factor is involved in the differentiation of second heart field-derived cardiac structures in chicken embryos. Dev. Dyn., 238: 2658–2669. doi: 10.1002/dvdy.22073
- Issue published online: 21 SEP 2009
- Article first published online: 24 AUG 2009
- Manuscript Accepted: 14 JUL 2009
- chicken embryos;
- heart development;
- epicardium-derived cells;
- second heart field
For the establishment of a fully functional septated heart, addition of myocardium from second heart field-derived structures is important. Platelet-derived growth factors (PDGFs) are known for their role in cardiovascular development. In this study, we aim to elucidate this role of PDGF-A, PDGF-C, and their receptor PDGFR-α. We analyzed the expression patterns of PDGF-A, -C, and their receptor PDGFR-α during avian heart development. A spatiotemporal pattern of ligands was seen with colocalization of the PDGFR-α. This was found in second heart field-derived myocardium as well as the proepicardial organ (PEO) and epicardium. Mechanical inhibition of epicardial outgrowth as well as chemical disturbance of PDGFR-α support a functional role of the ligands and the receptor in cardiac development. Developmental Dynamics 238:2658–2669, 2009. © 2009 Wiley-Liss, Inc.