Platelet-derived growth factor is involved in the differentiation of second heart field-derived cardiac structures in chicken embryos

Authors

  • Noortje A.M. Bax,

    1. Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
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  • Heleen Lie-Venema,

    1. Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
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  • Rebecca Vicente-Steijn,

    1. Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    2. Department of Cardiology, Leiden University Medical Center, Leiden, The Netherlands
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  • Steven B. Bleyl,

    1. Department of Pediatrics and Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah
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  • Nynke M.S. Van Den Akker,

    1. Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    Current affiliation:
    1. Department of Cardiology, CARIM, Maastricht University, Maastricht, the Netherlands
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  • Saskia Maas,

    1. Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
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  • Robert E. Poelmann,

    1. Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
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  • Adriana C. Gittenberger-de Groot

    Corresponding author
    1. Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands
    • Leiden University Medical Center, Department of Anatomy and Embryology, PO Box 9600, 2300 RC Leiden, The Netherlands
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Abstract

For the establishment of a fully functional septated heart, addition of myocardium from second heart field-derived structures is important. Platelet-derived growth factors (PDGFs) are known for their role in cardiovascular development. In this study, we aim to elucidate this role of PDGF-A, PDGF-C, and their receptor PDGFR-α. We analyzed the expression patterns of PDGF-A, -C, and their receptor PDGFR-α during avian heart development. A spatiotemporal pattern of ligands was seen with colocalization of the PDGFR-α. This was found in second heart field-derived myocardium as well as the proepicardial organ (PEO) and epicardium. Mechanical inhibition of epicardial outgrowth as well as chemical disturbance of PDGFR-α support a functional role of the ligands and the receptor in cardiac development. Developmental Dynamics 238:2658–2669, 2009. © 2009 Wiley-Liss, Inc.

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