Special Issue Research Article
A co-dependent requirement of xBcl9 and Pygopus for embryonic body axis development in Xenopus
Article first published online: 29 OCT 2009
Copyright © 2009 Wiley-Liss, Inc.
Special Issue: Special Issue on WNT Signaling in Development and Disease
Volume 239, Issue 1, pages 271–283, January 2010
How to Cite
Kennedy, M. W., Cha, S.-W., Tadjuidje, E., Andrews, P. G., Heasman, J. and Kao, K. R. (2010), A co-dependent requirement of xBcl9 and Pygopus for embryonic body axis development in Xenopus. Dev. Dyn., 239: 271–283. doi: 10.1002/dvdy.22133
- Issue published online: 15 DEC 2009
- Article first published online: 29 OCT 2009
- Manuscript Accepted: 7 SEP 2009
- Canadian Institutes of Health Research. Grant Number: MOP14928
- transcriptional regulation;
- Xenopus embryo;
- body axis development
The Wnt/β-catenin transcriptional activation complex requires the adapter protein Pygopus (Pygo), which links the basal transcription machinery to β-catenin, by its association with legless (Lgs)/ B-cell lymphoma-9 (Bcl9). Pygo was shown to be required for development in vertebrates, but the role of Lgs/Bcl9 is unknown. We identified an amphibian orthologue of Lgs/Bcl9, XBcl9, which interacted biochemically with Xβ-catenin and XPygo2. The body axis promoting ability of Xβ-catenin was diminished when residues required for its interaction with XBcl9 were mutated. In blastula embryos, XBcl9 was transiently preferentially expressed in nuclei of dorsoanterior cells and ectopically expressed XBcl9 required XPygo2 to localize to nuclei. Furthermore, while neither XBcl9 nor XPygo2 alone affected development when ectopically expressed, both were required to induce supernumerary axis and dorsal gene activation. Like XPygo2, depletion of maternal XBcl9 alone caused dorsal defects. These results indicated an essential role of the Pygo-Bcl9 duet in vertebrate body axis formation. Developmental Dynamics 239:271–283, 2010. © 2009 Wiley-Liss, Inc.