Dr. Li and A.M. Udager contributed equally to this work.
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Patterns & Phenotypes
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Dynamic patterning at the pylorus: Formation of an epithelial intestine–stomach boundary in late fetal life
Article first published online: 29 OCT 2009
DOI: 10.1002/dvdy.22134
Copyright © 2009 Wiley-Liss, Inc.
Additional Information
How to Cite
Li, X., Udager, A. M., Hu, C., Qiao, X. T., Richards, N. and Gumucio, D. L. (2009), Dynamic patterning at the pylorus: Formation of an epithelial intestine–stomach boundary in late fetal life. Dev. Dyn., 238: 3205–3217. doi: 10.1002/dvdy.22134
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Dr. Li and A.M. Udager contributed equally to this work.
Publication History
- Issue published online: 18 NOV 2009
- Article first published online: 29 OCT 2009
- Manuscript Accepted: 15 SEP 2009
Funded by
- The National Institute of Diabetes and Digestive and Kidney Diseases. Grant Numbers: R01 DK065850, F30 DK082144, P60 DK20572
Keywords:
- microarray analysis;
- intestinal identity;
- Hedgehog signaling;
- Wnt signaling;
- Gata3
Abstract
In the adult mouse, distinct morphological and transcriptional differences separate stomach from intestinal epithelium. Remarkably, the epithelial boundary between these two organs is literally one cell thick. This discrete junction is established suddenly and precisely at embryonic day (E) 16.5, by sharpening a previously diffuse intermediate zone. In the present study, we define the dynamic transcriptome of stomach, pylorus, and intestinal tissues between E14.5 and E16.5. We show that establishment of this boundary is concomitant with the induction of over a thousand genes in intestinal epithelium, and these gene products provide intestinal character. Hence, we call this process intestinalization. We identify specific transcription factors (Hnf4γ, Creb3l3, and Tcfec) and examine signaling pathways (Hedgehog and Wnt) that may play a role in this process. Finally, we define a unique expression domain at the pylorus itself and detect novel pylorus-specific patterns for the transcription factor Gata3 and the secreted protein nephrocan. Developmental Dynamics 238:3205–3217, 2009. © 2009 Wiley-Liss, Inc.