Whole genome microarray analysis of chicken embryo facial prominences

Authors

  • Marcela Buchtová,

    1. Department of Oral Health Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada
    Current affiliation:
    1. Institute of Animal Physiology and Genetics, v.v.i. Academy of Sciences of the Czech Republic, Brno, Czech Republic; Department of Anatomy, Histology and Embryology, University of Veterinary and Pharmaceutical Sciences
    Search for more papers by this author
  • Winston Patrick Kuo,

    1. Harvard-Catalyst, Laboratory for Innovative Translational Technologies, Harvard Medical School, Boston, Massachusetts
    2. Department of Developmental Biology, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, Massachusetts
    Search for more papers by this author
  • Suresh Nimmagadda,

    1. Department of Oral Health Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada
    Search for more papers by this author
  • Shari L. Benson,

    1. Harvard-Catalyst, Laboratory for Innovative Translational Technologies, Harvard Medical School, Boston, Massachusetts
    Current affiliation:
    1. Center for Molecular Orthopaedics, Harvard Medical School, 221 Longwood Ave Boston, MA 02115
    Search for more papers by this author
  • Poongodi Geetha-Loganathan,

    1. Department of Oral Health Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada
    Search for more papers by this author
  • Cairine Logan,

    1. Institute of Child and Maternal Health, Department of Cell Biology and Anatomy, University of Calgary, Calgary, Canada
    Search for more papers by this author
  • Timothy Au-Yeung,

    1. Department of Oral Health Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada
    Search for more papers by this author
  • Eric Chiang,

    1. Department of Oral Health Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada
    Search for more papers by this author
  • Katherine Fu,

    1. Department of Oral Health Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada
    Search for more papers by this author
  • Joy M. Richman

    Corresponding author
    1. Department of Oral Health Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada
    • Life Sciences Institute, 2350 Health Sciences Mall, UBC, Vancouver, BC V6T 1Z3, Canada
    Search for more papers by this author

Abstract

The face is one of the three regions most frequently affected by congenital defects in humans. To understand the molecular mechanisms involved, it is necessary to have a more complete picture of gene expression in the embryo. Here, we use microarrays to profile expression in chicken facial prominences, post neural crest migration and before differentiation of mesenchymal cells. Chip-wide analysis revealed that maxillary and mandibular prominences had similar expression profiles while the frontonasal mass chips were distinct. Of the 3094 genes that were differentially expressed in one or more regions of the face, a group of 56 genes was subsequently validated with quantitative polymerase chain reaction (QPCR) and a subset examined with in situ hybridization. Microarrays trends were consistent with the QPCR data for the majority of genes (81%). On the basis of QPCR and microarray data, groups of genes that characterize each of the facial prominences can be determined. Developmental Dynamics 239:574–591, 2010. © 2009 Wiley-Liss, Inc.

Ancillary