Patterns & Phenotypes

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Whole genome microarray analysis of chicken embryo facial prominences

  1. Marcela Buchtová1,†,
  2. Winston Patrick Kuo2,3,
  3. Suresh Nimmagadda1,
  4. Shari L. Benson2,‡,
  5. Poongodi Geetha-Loganathan1,
  6. Cairine Logan4,
  7. Timothy Au-Yeung1,
  8. Eric Chiang1,
  9. Katherine Fu1,
  10. Joy M. Richman1,*

Article first published online: 25 NOV 2009

DOI: 10.1002/dvdy.22135

Issue

Developmental Dynamics

Developmental Dynamics

Volume 239, Issue 2, pages 574–591, February 2010

Additional Information

How to Cite

Buchtová, M., Kuo, W. P., Nimmagadda, S., Benson, S. L., Geetha-Loganathan, P., Logan, C., Au-Yeung, T., Chiang, E., Fu, K. and Richman, J. M. (2010), Whole genome microarray analysis of chicken embryo facial prominences. Developmental Dynamics, 239: 574–591. doi: 10.1002/dvdy.22135

Author Information

  1. 1

    Department of Oral Health Sciences, Life Sciences Institute, University of British Columbia, Vancouver, Canada

  2. 2

    Harvard-Catalyst, Laboratory for Innovative Translational Technologies, Harvard Medical School, Boston, Massachusetts

  3. 3

    Department of Developmental Biology, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, Massachusetts

  4. 4

    Institute of Child and Maternal Health, Department of Cell Biology and Anatomy, University of Calgary, Calgary, Canada

  1. †

    Institute of Animal Physiology and Genetics, v.v.i. Academy of Sciences of the Czech Republic, Brno, Czech Republic; Department of Anatomy, Histology and Embryology, University of Veterinary and Pharmaceutical Sciences

  2. ‡

    Center for Molecular Orthopaedics, Harvard Medical School, 221 Longwood Ave Boston, MA 02115

*Life Sciences Institute, 2350 Health Sciences Mall, UBC, Vancouver, BC V6T 1Z3, Canada

Publication History

  1. Issue published online: 22 JAN 2010
  2. Article first published online: 25 NOV 2009
  3. Manuscript Accepted: 20 SEP 2009

Funded by

  • Canadian Institutes of Health Research. Grant Number: MOP49431
  • the Grant Agency of Czech Republic. Grant Number: 304/09/0725

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Keywords:

  • pharyngeal arch;
  • mandibular arch;
  • maxillary prominence;
  • frontonasal mass;
  • array;
  • craniofacial;
  • avian;
  • gallus;
  • ASCL1;
  • TAGLN3;
  • NP25;
  • ID1;
  • MEIS2;
  • SP8;
  • MAB21L2

Abstract

The face is one of the three regions most frequently affected by congenital defects in humans. To understand the molecular mechanisms involved, it is necessary to have a more complete picture of gene expression in the embryo. Here, we use microarrays to profile expression in chicken facial prominences, post neural crest migration and before differentiation of mesenchymal cells. Chip-wide analysis revealed that maxillary and mandibular prominences had similar expression profiles while the frontonasal mass chips were distinct. Of the 3094 genes that were differentially expressed in one or more regions of the face, a group of 56 genes was subsequently validated with quantitative polymerase chain reaction (QPCR) and a subset examined with in situ hybridization. Microarrays trends were consistent with the QPCR data for the majority of genes (81%). On the basis of QPCR and microarray data, groups of genes that characterize each of the facial prominences can be determined. Developmental Dynamics 239:574–591, 2010. © 2009 Wiley-Liss, Inc.

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