Loss of Wnt8b has no overt effect on hippocampus development but leads to altered Wnt gene expression levels in dorsomedial telencephalon

Authors

  • Vassiliki Fotaki,

    1. Genes and Development Group, Centre for Integrative Physiology, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, United Kingdom
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    • Drs. Fotaki, Larralde, and Zeng contributed equally to this work.

  • Osmany Larralde,

    1. Genes and Development Group, Centre for Integrative Physiology, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, United Kingdom
    Current affiliation:
    1. Scottish National Blood Transfusion Service, Liberton, Edinburgh, UK.
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    • Drs. Fotaki, Larralde, and Zeng contributed equally to this work.

  • Shaoju Zeng,

    1. Genes and Development Group, Centre for Integrative Physiology, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, United Kingdom
    2. Key Laboratory for Cell Proliferation and Regulation Biology, Beijing Normal University, China
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  • David McLaughlin,

    1. Genes and Development Group, Centre for Integrative Physiology, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, United Kingdom
    Current affiliation:
    1. UCD School of Biomolecular and Biomedical Science, University College Dublin, Ireland.
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  • Jennifer Nichols,

    1. Institute for Stem Cell Research, University of Edinburgh, King's Buildings, Edinburgh, United Kingdom
    Current affiliation:
    1. Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK.
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  • David J. Price,

    1. Genes and Development Group, Centre for Integrative Physiology, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, United Kingdom
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  • Thomas Theil,

    1. Genes and Development Group, Centre for Integrative Physiology, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, United Kingdom
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  • John O. Mason

    Corresponding author
    1. Genes and Development Group, Centre for Integrative Physiology, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, United Kingdom
    • Genes and Development Group, Centre for Integrative Physiology, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK
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Abstract

Wnt signalling proteins regulate many aspects of animal development. We have investigated the function of mouse Wnt8b during forebrain development. Wnt8b is expressed in a highly restricted pattern including the prospective hippocampus and hypothalamus. Mutant mice lacking Wnt8b are viable and healthy. The size and morphology of the hippocampus appeared normal in mutant embryos and adults, and we found no evidence of hypothalamic defects in mutants. Wnt8b is also expressed in the neurogenic region of the adult dentate gyrus, however, cell proliferation was unchanged in Wnt8b−/− mutants. Mutant embryos did, however, display altered levels of expression of other Wnt genes normally expressed in forebrain. The spatial expression patterns of other Wnt genes and the overall level of canonical Wnt activity were indistinguishable from wild-types. Thus, loss of Wnt8b does not give rise to an overt morphological phenotype, but does affect expression levels of other Wnts in developing forebrain. Developmental Dynamics 239:284–296, 2010. © 2009 Wiley-Liss, Inc.

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