Fusion of uniluminal vascular spheroids: A model for assembly of blood vessels
Article first published online: 13 NOV 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 239, Issue 2, pages 398–406, February 2010
How to Cite
Fleming, P. A., Argraves, W. S., Gentile, C., Neagu, A., Forgacs, G. and Drake, C. J. (2010), Fusion of uniluminal vascular spheroids: A model for assembly of blood vessels. Dev. Dyn., 239: 398–406. doi: 10.1002/dvdy.22161
- Issue published online: 22 JAN 2010
- Article first published online: 13 NOV 2009
- Manuscript Accepted: 5 OCT 2009
- NIH. Grant Numbers: HL57375, HL80168, HL061873, HL095067
- National Science Foundation. Grant Number: EEC-0244045 and FIBR-0526854
- tissue liquidity;
- blood vessels;
- dorsal aorta;
- differential adhesion;
We evaluated the self-assembly properties of uniluminal vascular spheroids having outer layers of vascular smooth muscle cells and a contiguous inner layer of endothelial cells lining a central lumen. We showed that while pairs of uniluminal vascular spheroids suspended in culture medium fused to form a larger diameter spheroidal structure, spheroids in collagen hydrogels formed elongated structures. These findings highlight the potential use of uniluminal vascular spheroids as modules to engineer blood vessels. We also demonstrate that uniluminal vascular spheroid fusion conforms to models describing the coalescence of liquid drops. Furthermore, the fusion of uniluminal vascular spheroids in vitro closely resembled the in vivo process by which the descending aorta forms from the fusion of the paired dorsal aortae during embryonic development. Together, the findings indicate that tissue liquidity underlies uniluminal vascular spheroid fusion and that in vivo anastomosis of blood vessels may involve a similar mechanism. Developmental Dynamics 239:398–406, 2010. © 2009 Wiley-Liss, Inc.