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Visualization of myelination in GFP-transgenic zebrafish

  1. Seung-Hyun Jung1,†,
  2. Suhyun Kim2,†,
  3. Ah-Young Chung2,
  4. Hyun-Taek Kim1,
  5. Ju-Hoon So1,
  6. Jaeho Ryu3,
  7. Hae-Chul Park2,*,
  8. Cheol-Hee Kim1,*

Article first published online: 13 NOV 2009

DOI: 10.1002/dvdy.22166

Issue

Developmental Dynamics

Developmental Dynamics

Volume 239, Issue 2, pages 592–597, February 2010

Additional Information

How to Cite

Jung, S.-H., Kim, S., Chung, A.-Y., Kim, H.-T., So, J.-H., Ryu, J., Park, H.-C. and Kim, C.-H. (2010), Visualization of myelination in GFP-transgenic zebrafish. Dev. Dyn., 239: 592–597. doi: 10.1002/dvdy.22166

Author Information

  1. 1

    Department of Biology and GRAST, Chungnam National University, Daejeon, Republic of Korea

  2. 2

    Graduate School of Medicine, Korea University, Ansan, Gyeonggido, Republic of Korea

  3. 3

    Genomic Design Bioengineering Company, Daejeon, Republic of Korea

  1. †

    S.-H. Jung and S. Kim contributed equally to this work.

*Hae-Chul Park, Graduate School of Medicine, Korea University, Ansan, Gyeonggido, Republic of Korea

  1. †

    S.-H. Jung and S. Kim contributed equally to this work.

Publication History

  1. Issue published online: 22 JAN 2010
  2. Article first published online: 13 NOV 2009
  3. Manuscript Accepted: 6 OCT 2009

Funded by

  • Korea Health 21 R&D Project funded by Ministry of Health and Welfare. Grant Number: A084909
  • 21C Frontier Functional Human Genome Project. Grant Number: FG09-42-1
  • KOSEF. Grant Number: Vascular System Research Grant

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Keywords:

  • oligodendrocytes;
  • myelination;
  • transgenic zebrafish;
  • mbp

Abstract

The insulation of axons in the vertebrate nervous system by myelin is essential for efficient axonal conduction. Myelination disruption and remyelination failure can cause human diseases, such as multiple sclerosis and hereditary myelin diseases. However, despite progress in understanding myelination regulation, many important questions remain unanswered. To investigate the mechanisms underlying myelination in vivo, we generated transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under the control of the mbp promoter. This transgenic fish displayed faithful EGFP expression in oligodendrocytes and Schwann cells in embryonic and adult zebrafish. Interestingly, although myelination progressed continuously in the postembryonic central nervous system, some of the spinal cord regions were filled with unmyelinated axons even in the adult spinal cord, suggesting functional differences between myelinated and unmyelinated axons. Our results suggest that this transgenic zebrafish could be a valuable animal model to study oligodendrocyte differentiation and myelination in vivo. Developmental Dynamics 239:592–597, 2010. © 2009 Wiley-Liss, Inc.

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