S.-H. Jung and S. Kim contributed equally to this work.
Patterns & Phenotypes
Visualization of myelination in GFP-transgenic zebrafish
Article first published online: 13 NOV 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 239, Issue 2, pages 592–597, February 2010
How to Cite
Jung, S.-H., Kim, S., Chung, A.-Y., Kim, H.-T., So, J.-H., Ryu, J., Park, H.-C. and Kim, C.-H. (2010), Visualization of myelination in GFP-transgenic zebrafish. Dev. Dyn., 239: 592–597. doi: 10.1002/dvdy.22166
- Issue published online: 22 JAN 2010
- Article first published online: 13 NOV 2009
- Manuscript Accepted: 6 OCT 2009
- Korea Health 21 R&D Project funded by Ministry of Health and Welfare. Grant Number: A084909
- 21C Frontier Functional Human Genome Project. Grant Number: FG09-42-1
- KOSEF. Grant Number: Vascular System Research Grant
- transgenic zebrafish;
The insulation of axons in the vertebrate nervous system by myelin is essential for efficient axonal conduction. Myelination disruption and remyelination failure can cause human diseases, such as multiple sclerosis and hereditary myelin diseases. However, despite progress in understanding myelination regulation, many important questions remain unanswered. To investigate the mechanisms underlying myelination in vivo, we generated transgenic zebrafish expressing enhanced green fluorescent protein (EGFP) under the control of the mbp promoter. This transgenic fish displayed faithful EGFP expression in oligodendrocytes and Schwann cells in embryonic and adult zebrafish. Interestingly, although myelination progressed continuously in the postembryonic central nervous system, some of the spinal cord regions were filled with unmyelinated axons even in the adult spinal cord, suggesting functional differences between myelinated and unmyelinated axons. Our results suggest that this transgenic zebrafish could be a valuable animal model to study oligodendrocyte differentiation and myelination in vivo. Developmental Dynamics 239:592–597, 2010. © 2009 Wiley-Liss, Inc.