Comparative evaluation of human embryonic stem cell lines derived from zygotes with normal and abnormal pronuclei

Authors

  • Qing Huan,

    1. Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China
    2. Key Laboratory of Reproductive Medicine, Shandong Province, People's Republic of China
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  • Xuan Gao,

    1. Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China
    2. Key Laboratory of Reproductive Medicine, Shandong Province, People's Republic of China
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  • Yan Wang,

    1. Plastic Surgical Department of Qianfoshan Hospital Affiliated to Shandong University, Jinan, People's Republic of China
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  • Yun Shen,

    1. Central laboratory, Shandong Provincial Hospital, Jinan, People's Republic of China
    2. Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, People's Republic of China
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  • Wu Ma,

    1. Stem Cell Center, Developmental Biology Collection, American Type Culture Collection (ATCC), Manassas, Virginia
    Current affiliation:
    1. iPierian Inc., 951 Gateway Blvd., South San Francisco, CA 94080
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  • Zi-Jiang Chen

    Corresponding author
    1. Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China
    2. Key Laboratory of Reproductive Medicine, Shandong Province, People's Republic of China
    3. School of Medicine, Department of MMI, University of Missouri-Columbia, Missouri
    • Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jing-5-Wei-7 Road, Jinan, 250021, People's Republic of China
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Abstract

Human embryonic stem (hES) cell lines have been derived from normally or abnormally fertilized zygotes. However, the similar and different properties of these two types of hES cell lines are not well-known. To address this question, we generated nine hES cell lines from zygotes containing normal (2PN) and abnormal (0PN, 1PN, 3PN) pronuclei. A side-by-side comparison showed that all cell lines exhibited distinct identity and karyotypical stability. They expressed similar “stemness” markers and alkaline phosphatase activity and differentiated into three embryonic germ lineages in embryoid bodies and teratomas. Under neural differentiation-promoting conditions, they were directed into neural progenitors and neurons. However, a variation in cell cycle and the relative abundance of gene expression of undifferentiated and differentiated markers were observed. These variations were also seen among individually derived normal hES cell lines. Thus, normal hES cell lines can be developed from fertilized zygotes with abnormal pronuclei usually excluded from clinical use. Developmental Dynamics 239:425–438, 2010. © 2009 Wiley-Liss, Inc.

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