Enucleation of feeder cells and egg cells with psoralens

Authors

  • Thomas J. McGarry,

    Corresponding author
    1. Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, Illinois
    2. Department of Medicine, Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, Illinois
    3. Department of Cell and Molecular Biology, Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, Illinois
    • Feinberg Cardiovascular Research Institute, 303 E. Chicago Avenue, Tarry 14-721, Chicago, IL 60611
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  • Michael Bonaguidi,

    1. Department of Neurology, Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, Illinois
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  • Ljuba Lyass,

    1. Department of Neurology, Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, Illinois
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  • John A. Kessler,

    1. Department of Neurology, Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, Illinois
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  • Jason M. Bodily,

    1. Microbiology and Immunology, Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, Illinois
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  • Lynn Doglio

    1. Center for Genetic Medicine, Feinberg School of Medicine, Northwestern University, Chicago. Illinois
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Abstract

GFP-expressing Xenopus tadpole made by transplanting a nucleus from a transgenic CMV-GFP blastula into an enucleated GFP(−) egg. The egg was enucleated using a new method, treatment with psoralens and ultraviolet light. See McGarry et al., Developmental Dynamics 238:2614–2621, 2009. © 2009 Wiley-Liss, Inc.

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