SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
DVDY_22180_sm_SuppFig1.tif3454KSupp. Fig. 1. Timeline of apoptosis revealed by pHMA. A version of the images shown in Figure 7A with a semi-transparent overlay. The dying cell is shaded red and the engulfing cell is shaded blue in the fluorescence images. The dashed lines in the cartoons indicate vesicles that are out of focus.
DVDY_22180_sm_SuppMovie1.mov1902KSupp. Movie 1. pHMA fluorescence overlaid with transmitted light time-lapse recording of cell a in Figure 2C and D cell with cortical pHMA localization and neutral pH.
DVDY_22180_sm_SuppMovie2.mov416KSupp. Movie 2. pHMA fluorescence overlaid with transmitted light time-lapse recording of cell b in Figure 2E–G, which is likely necrotic.
DVDY_22180_sm_SuppMovie3.mov259KSupp. Movie 3. pHMA fluorescence overlaid with transmitted light time-lapse recording of engulfed cell c in Figure 2H–K.
DVDY_22180_sm_SuppMovie4.mov1272KSupp. Movie 4. pHMA fluorescence overlaid with transmitted light time-lapse recording of pHMA-expressing cells d and e in Figure 2L–O. The cell that has some red has already engulfed something. Two other cells acidify slightly (green [RIGHTWARDS ARROW] yellow) and are engulfed by the daughters of the red cell. They quickly acidify after engulfment (yellow [RIGHTWARDS ARROW] red).
DVDY_22180_sm_SuppMovie5.mov1818KSupp. Movie 5. Time-lapse recording of the wild-type embryo injected with AO and VGAL that was used for Figure 3A.
DVDY_22180_sm_SuppMovie6.mov1365KSupp. Movie 6. Time-lapse recording of the UAS-pHMA embryo injected with GAL4VP16 that was used for Figure 3B.
DVDY_22180_sm_SuppMovie7.mov2768KSupp. Movie 7. Time-lapse recording of the en-GAL4/+; UAS-pHMA/+ embryo that was used for Figure 4A–D.
DVDY_22180_sm_SuppMovie8.mov2127KSupp. Movie 8. Time-lapse recording of the UAS-rpr, UAS-W/+; en-GAL4/+; UAS-pHMA/+ embryo that was used for Figure 4E–H.
DVDY_22180_sm_SuppMovie9.mov1551KSupp. Movie 9. Time-lapse recording of the dorsal closure in UAS-pHMA;H99/H99 embryo that was used for Figure 6B, right column. The R470/410 signal remains uniform throughout dorsal closure, indicating that pHMA is not acidified.
DVDY_22180_sm_SuppMovie10.mov1132KSupp. Movie 10. Time-lapse recording of the en-GAL4, UAS-pHMA wing disc from late third instar larva that was used for Figure 9A–A″ and B–B″. A 5-hr recording was captured at 1-min intervals.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.