The function and regulation of TBX22 in avian frontonasal morphogenesis
Article first published online: 23 DEC 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 239, Issue 2, pages 458–473, February 2010
How to Cite
Higashihori, N., Buchtová, M. and Richman, J. M. (2010), The function and regulation of TBX22 in avian frontonasal morphogenesis. Dev. Dyn., 239: 458–473. doi: 10.1002/dvdy.22182
- Issue published online: 22 JAN 2010
- Article first published online: 23 DEC 2009
- Manuscript Accepted: 4 NOV 2009
- Alpha Omega Foundation
The frontonasal mass gives rise to the facial midline and fuses with the maxillary prominence to form the upper lip. Here we focus on the regulation and function of TBX22, a repressor dynamically expressed in the frontonasal mass. Both FGF and Noggin (a BMP antagonist) strongly induce gTBX22, however, each has opposite effects on morphogenesis - Noggin inhibits whereas FGF stimulates growth. To determine whether TBX22 mediates these effects, we used retroviruses to locally increase expression levels. RCAS::hTBX22 decreased proliferation, reduced expression of MSX2 and DLX5 and caused cleft lip. Decreased levels of endogenous gTBX22 were also observed but were not the primary cause of the phenotype as determined in rescue experiments. Our data suggest that genetic or environmental insults such as those affecting the BMP pathway could lead to a gain-of-function of TBX22 and predispose an individual to cleft lip. Developmental Dynamics 239:458–473, 2010. © 2009 Wiley-Liss, Inc.