Regulation of Sox9 by Sonic Hedgehog (Shh) is essential for patterning and formation of tracheal cartilage
Version of Record online: 23 DEC 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 239, Issue 2, pages 514–526, February 2010
How to Cite
Park, J., Zhang, J. J.R., Moro, A., Kushida, M., Wegner, M. and Kim, P. C.W. (2010), Regulation of Sox9 by Sonic Hedgehog (Shh) is essential for patterning and formation of tracheal cartilage. Dev. Dyn., 239: 514–526. doi: 10.1002/dvdy.22192
- Issue online: 22 JAN 2010
- Version of Record online: 23 DEC 2009
- Manuscript Accepted: 17 NOV 2009
- Canadian Institute of Health Research (CIHR). Grant Number: MOP-86628
Additional Supporting Information may be found in the online version of this article.
|DVDY_22192_sm_SuppFig1.tif||3713K||Supp. Fig. S1. Continuous expression of Sox9 in WT trachea after chondrogenesis. A: Immunostaining of Sox9 on E17.5 wild-type trachea shows its clear expression in cartilages (arrow, 100×). B: Sox9 expression is undetectable in mesenchyme of the Shh−/− tracheo-esophageal tube at E17.5 (arrow, 100×). Blue, DAPI background; red, Sox9; Tr, trachea; TE, tracheo-esophageal tube.|
|DVDY_22192_sm_SuppFig2.tif||3932K||Supp. Fig. S2. Bmp4 and Noggin expression in trachea of WT and Shh−/− embryos. A,B: Immunofluorescent staining of Bmp4 on E11.5 wild-type tracheal (A) and Shh−/− tracheo-esophageal tube sections (B) show the significant loss of Bmp4 expression in Shh−/− foregut (B), and its expression in wild-type ventral tracheal mesenchyme (A) (200×). C,D: Immunofluorescent staining of Noggin on wild-type (C) and Shh−/− sections (D) show Noggin expression in dorsal mesenchyme of wild-type trachea (C), which is also significantly reduced, if present, in the Shh−/− tracheo-esophageal tube (D) (200×).|
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