SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
DVDY_22192_sm_SuppFig1.tif3713KSupp. Fig. S1. Continuous expression of Sox9 in WT trachea after chondrogenesis. A: Immunostaining of Sox9 on E17.5 wild-type trachea shows its clear expression in cartilages (arrow, 100×). B: Sox9 expression is undetectable in mesenchyme of the Shh−/− tracheo-esophageal tube at E17.5 (arrow, 100×). Blue, DAPI background; red, Sox9; Tr, trachea; TE, tracheo-esophageal tube.
DVDY_22192_sm_SuppFig2.tif3932KSupp. Fig. S2. Bmp4 and Noggin expression in trachea of WT and Shh−/− embryos. A,B: Immunofluorescent staining of Bmp4 on E11.5 wild-type tracheal (A) and Shh−/− tracheo-esophageal tube sections (B) show the significant loss of Bmp4 expression in Shh−/− foregut (B), and its expression in wild-type ventral tracheal mesenchyme (A) (200×). C,D: Immunofluorescent staining of Noggin on wild-type (C) and Shh−/− sections (D) show Noggin expression in dorsal mesenchyme of wild-type trachea (C), which is also significantly reduced, if present, in the Shh−/− tracheo-esophageal tube (D) (200×).

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.