Expression and regulation of ANTXR1 in the chick embryo

Authors

  • David Herrmann,

    1. Developmental Biology Unit, Institute of Biology I, Faculty of Biology, University of Freiburg, Freiburg, Germany
    2. Spemann Graduate School of Biology and Medicine, University of Freiburg, Freiburg, Germany
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    • D. Herrmann and Ferrer-Vaquer contributed equally to this work.

  • Anna Ferrer-Vaquer,

    1. Developmental Biology Unit, Institute of Biology I, Faculty of Biology, University of Freiburg, Freiburg, Germany
    Current affiliation:
    1. Sloan-Kettering Institute, Developmental Biology Program, New York, NY 10065
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    • D. Herrmann and Ferrer-Vaquer contributed equally to this work.

  • Christian Lahsnig,

    1. Institute of Molecular Pathology, Vienna, Austria
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  • Nicole Firnberg,

    1. Institute of Molecular Pathology, Vienna, Austria
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  • Andreas Leibbrandt,

    1. Institute of Molecular Pathology, Vienna, Austria
    Current affiliation:
    1. Marinomed Biotechnologie GmbH, Veterinärplatz 1, 1210 Vienna, Austria
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  • Annette Neubüser

    Corresponding author
    1. Developmental Biology Unit, Institute of Biology I, Faculty of Biology, University of Freiburg, Freiburg, Germany
    • Developmental Biology Unit, Institute of Biology I, Faculty of Biology, University of Freiburg, Hauptstrasse 1, D-79104 Freiburg, Germany
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Abstract

Anthrax Toxin Receptor 1 (ANTXR1; also known as Tumor Endothelial Marker 8, TEM8) is one of several genes that was recently found to be up-regulated in tumor-associated endothelial cells. In vitro, the protein can link extracellular matrix components with the actin cytoskeleton to promote cell adhesion and cell spreading. Both, ANTXR1 and the closely related ANTXR2 can bind anthrax toxin and interact with lipoprotein receptor-related protein 5 and 6, which also work as coreceptors in the WNT signaling pathway. Here, we report the cloning of chick ANTXR1 from a suppression subtractive hybridization screen for fibroblast growth factor (FGF) -inducible genes in chicken embryonic facial mesenchyme. We show that chicken ANTXR1 is dynamically expressed throughout embryogenesis, starting from Hamburger and Hamilton stage 10. Furthermore, we demonstrate that FGF signaling is sufficient, but not necessary, to induce ANTXR1 expression in chicken facial mesenchyme. Developmental Dynamics 239:680–687, 2010. © 2009 Wiley-Liss, Inc.

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