Federico Bussolino, Marco Arese, and Franco Cotelli contributed equally to the project.
Patterns & Phenotypes
Characterization of the neuroligin gene family expression and evolution in zebrafish
Version of Record online: 23 DEC 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 239, Issue 2, pages 688–702, February 2010
How to Cite
Rissone, A., Sangiorgio, L., Monopoli, M., Beltrame, M., Zucchi, I., Bussolino, F., Arese, M. and Cotelli, F. (2010), Characterization of the neuroligin gene family expression and evolution in zebrafish. Dev. Dyn., 239: 688–702. doi: 10.1002/dvdy.22196
- Issue online: 22 JAN 2010
- Version of Record online: 23 DEC 2009
- Manuscript Accepted: 20 NOV 2009
- Associazione Italiana per la Ricerca sul Cancro (AIRC)
- Grant Sponsor: Fondazione CRT-Torino
- Grant Sponsor: “Ministero della Salute”
- Grant Sponsor: Fondazione Cariplo. Grant Number: 2006.0807
- Progetto Cariplo N.O.B.E.L.
- Regione Piemonte (Ricerca Scientifica Applicata 2004, A17)
- alternative splicing;
Neuroligins constitute a family of transmembrane proteins localized at the postsynaptic side of both excitatory and inhibitory synapses of the central nervous system. They are involved in synaptic function and maturation and recent studies have linked mutations in specific human Neuroligins to mental retardation and autism. We isolated the human Neuroligin homologs in Danio rerio. Next, we studied their gene structures and we reconstructed the evolution of the Neuroligin genes across vertebrate phyla. Using reverse-transcriptase polymerase chain reaction, we analyzed the expression and alternative splicing pattern of each gene during zebrafish embryonic development and in different adult organs. By in situ hybridization, we analyzed the temporal and spatial expression pattern during embryonic development and larval stages and we found that zebrafish Neuroligins are expressed throughout the nervous system. Globally, our results indicate that, during evolution, specific subfunctionalization events occurred within paralogous members of this gene family in zebrafish. Developmental Dynamics 239:688–702, 2010. © 2009 Wiley-Liss, Inc.