A.V. Sánchez-Sánchez, E. Camp, and A. García-España contributed equally to this work.
Patterns & Phenotypes
Medaka Oct4 is expressed during early embryo development, and in primordial germ cells and adult gonads
Version of Record online: 23 DEC 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 239, Issue 2, pages 672–679, February 2010
How to Cite
Sánchez-Sánchez, A. V., Camp, E., García-España, A., Leal-Tassias, A. and Mullor, J. L. (2010), Medaka Oct4 is expressed during early embryo development, and in primordial germ cells and adult gonads. Dev. Dyn., 239: 672–679. doi: 10.1002/dvdy.22198
- Issue online: 22 JAN 2010
- Version of Record online: 23 DEC 2009
- Manuscript Accepted: 14 NOV 2009
- Spanish MEC. Grant Numbers: BFU2005-09186/BFI, BFU2008-04624/BFI
- Valencian Health Conselleria. Grant Numbers: GV05/221, ACOMP06/145
- Spanish ISCIII: Regenerative Program of the Valencian Community
- FIS. Grant Numbers: 02/3003, PI070789
Oct4 is a crucial transcription factor for controlling pluripotency in embryonic stem cells and the epiblast of mouse embryos. We have characterized the expression pattern of medaka (Oryzias latipes) Ol-Oct4 during embryonic development and in the adult gonads. Genomic analysis showed that Ol-Oct4 is the ortholog of zebrafish spg/pou2. However, their expression patterns are not the same, suggesting that Oct4 may play different roles in zebrafish and medaka. Using specific antibodies for the Ol-Oct4 protein, we showed that Ol-Oct4 is also expressed in primordial germ cells, in the spermatogonia (male germ stem cells), and during different stages of oocyte development. These results suggest that Ol-Oct4 plays a post-embryonic role in the maturing gonads and gametes. The Ol-Oct4 mRNA and protein expression patterns are similar to those of mammalian Oct4 and introduce medaka fish as a valid model for the functional and evolutionary study of pluripotency genes in vivo. Developmental Dynamics 239:672–679, 2010. © 2009 Wiley-Liss, Inc.