The iguana/DZIP1 protein is a novel component of the ciliogenic pathway essential for axonemal biogenesis
Article first published online: 15 DEC 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 239, Issue 2, pages 527–534, February 2010
How to Cite
Tay, S. Y., Yu, X., Wong, K. N., Panse, P., Ng, C. P. and Roy, S. (2010), The iguana/DZIP1 protein is a novel component of the ciliogenic pathway essential for axonemal biogenesis. Dev. Dyn., 239: 527–534. doi: 10.1002/dvdy.22199
- Issue published online: 22 JAN 2010
- Article first published online: 15 DEC 2009
- Manuscript Accepted: 19 NOV 2009
- Institute of Molecular and Cell Biology and the Agency for Science, Technology and Research of Singapore
- basal body;
Cilia play important roles in many developmental and physiological processes. However, the genetic and cell biological control of ciliogenesis remains poorly understood. Here, we show that the zebrafish iguana gene is required for differentiation of primary cilia. iguana encodes a zinc finger and coiled-coil containing protein, previously implicated in Hedgehog signaling. We now argue that aberrant Hedgehog activity in iguana -deficient zebrafish arises from their profound lack of primary cilia. By contrast, the requirement of iguana for motile cilia formation is less obligatory. In the absence of iguana function, basal bodies can migrate to the cell surface and appear to engage with the apical membrane. However, formation of ciliary pits and axonemal outgrowth is completely inhibited. Iguana localizes to the base of primary and motile cilia, in the immediate vicinity or closely associated with the basal bodies. These findings identify the Iguana protein as a novel and critical component of ciliogenesis. Developmental Dynamics 239:527–534, 2010. © 2009 Wiley-Liss, Inc.