Role of sperm αvβ3 integrin in mouse fertilization

Authors

  • Céline Chalas Boissonnas,

    1. Université Paris Descartes, Laboratoire Interaction Gamétique et Fusion des Membranes, EA 2717, Institut Alfred Jost, Paris, France
    2. Service d'Histologie Embryologie Biologie de la Reproduction, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, France
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  • Debbie Montjean,

    1. Institut Pasteur, Human Developmental Genetics, Paris, France
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  • Corinne Lesaffre,

    1. Université Paris Descartes, Laboratoire Interaction Gamétique et Fusion des Membranes, EA 2717, Institut Alfred Jost, Paris, France
    2. Inserm U567, Department of Genetics and Development, Cochin Institute, Paris, France
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  • Jana Auer,

    1. Université Paris Descartes, Laboratoire Interaction Gamétique et Fusion des Membranes, EA 2717, Institut Alfred Jost, Paris, France
    2. Inserm U567, Department of Genetics and Development, Cochin Institute, Paris, France
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  • Daniel Vaiman,

    1. Inserm U567, Department of Genetics and Development, Cochin Institute, Paris, France
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  • Jean-Philippe Wolf,

    Corresponding author
    1. Université Paris Descartes, Laboratoire Interaction Gamétique et Fusion des Membranes, EA 2717, Institut Alfred Jost, Paris, France
    2. Service d'Histologie Embryologie Biologie de la Reproduction, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, France
    • Université Paris Descartes, Laboratoire Interaction Gamétique et Fusion des Membranes, EA 2717, Institut Alfred Jost, 24 rue du Fbg Saint Jacques, 75014 Paris
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  • Ahmed Ziyyat

    1. Université Paris Descartes, Laboratoire Interaction Gamétique et Fusion des Membranes, EA 2717, Institut Alfred Jost, Paris, France
    2. Service d'Histologie Embryologie Biologie de la Reproduction, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, France
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Abstract

Oocyte integrins have been described as essential for fertilization. But this concept has been challenged by deletion experiments. Recently, we have shown that sperm integrin α6β1 plays a determinant role in mouse gamete interaction. In this study, we demonstrate the presence of αvβ3 integrin by Western blot and immunofluorescence on the sperm membrane. Oocytes and/or sperm preincubations with anti-αv or anti-β3 antibodies were performed before in vitro fertilization on cumulus-intact and zona-free egg assays. We observed inhibitory effects on the fusion process mostly by means of sperm function. An antibody directed against vitronectin inhibited gametes fusion, whereas the presence of exogenous vitronectin increased its efficiency. We suggest that vitronectin (on multimeric forms) can play a first nonspecific link corresponding to loosely bound spermatozoa to oocyte and that this link could be mediated by means of oocyte proteoglycans or integrins, and sperm αvβ3 integrin. Developmental Dynamics 239:773–783, 2010. © 2010 Wiley-Liss, Inc.

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