Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs
Article first published online: 23 FEB 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 4, pages 1076–1088, April 2010
How to Cite
Hozumi, A., Kawai, N., Yoshida, R., Ogura, Y., Ohta, N., Satake, H., Satoh, N. and Sasakura, Y. (2010), Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs. Dev. Dyn., 239: 1076–1088. doi: 10.1002/dvdy.22254
- Issue published online: 16 MAR 2010
- Article first published online: 23 FEB 2010
- Manuscript Accepted: 25 JAN 2010
- NIG Cooperative Research Program. Grant Numbers: 2008-B02, 2009-A18
- National Bioresource Project
Transgenesis with transposons is an important technique for studying genetic functions. In the ascidian Ciona intestinalis, methods for germline transformation with the Tc1/mariner transposon Minos have been established. A system to remobilize a single Minos copy in the genome is needed to refine this transgenic technique. In this study, such an experimental system was established with a transgenic line expressing Minos transposase in eggs. In the eggs of a double transgenic animal from a cross between the egg transposase line and a transgenic line having a single Minos insertion, the transposon was transposed into new positions of the Ciona genome, thus creating new insertions. Some of the new insertions caused enhancer detection. The majority of the new insertion sites were mapped on different chromosomes from that of the transposon donor. This characteristic of Minos is in contrast to that of the Sleeping Beauty transposon, which causes frequent intrachromosomal transposition. Developmental Dynamics 239:1076–1088, 2010. © 2010 Wiley-Liss, Inc.