• Open Access

Fusion of uniluminal vascular spheroids: A model for assembly of blood vessels

Authors

  • Paul A. Fleming,

    1. Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, South Carolina
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  • W. Scott Argraves,

    1. Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, South Carolina
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  • Carmine Gentile,

    1. Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, South Carolina
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  • Adrian Neagu,

    1. Victor Babes University of Medicine and Pharmacy Timisoara, Timisoara, Romania
    2. Department of Physics and Biology, University of Missouri Columbia, Missouri
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  • Gabor Forgacs,

    1. Department of Physics and Biology, University of Missouri Columbia, Missouri
    2. Department of Biological Sciences, University of Missouri Columbia, Missouri
    3. Department of Biomedical Engineering, University of Missouri Columbia, Missouri
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  • Christopher J. Drake

    Corresponding author
    1. Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, South Carolina
    • Department of Cell Biology and Anatomy, Charleston, SC 29425
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Abstract

Blood vessel formation via vascular fusion. When placed into hanging drop culture, five uniluminal vascular spheroids fuse to form a single, larger diameter spheroid with an outer layer of smooth muscle alpha actin positive cells (red) and an inner PECAM-1 positive endothelium (green) surrounding a large central lumen. From Fleming et al., Developmental Dynamics 239:398–406, 2010.

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