Differential time course of FSH/FSH receptor complex endocytosis within sertoli and germ cells during rat testis development

Authors

  • Dominique Segretain,

    1. INSERM U 895, Université Paris Descartes, Paris, France
    2. Université de Nice Sophia Antipolis, Nice, France
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  • Jérome Gilleron,

    1. INSERM U 895, Université Paris Descartes, Paris, France
    2. Université de Nice Sophia Antipolis, Nice, France
    Current affiliation:
    1. Zerial Laboratory, Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01309, Dresden, Germany
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  • Diane Carette,

    1. INSERM U 895, Université Paris Descartes, Paris, France
    2. Université de Nice Sophia Antipolis, Nice, France
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  • Jean-Pierre Denizot,

    1. C.N.R.S., Unité de Neurosciences Intégratives et Computationnelles, Gif-sur-Yvette, France
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  • Georges Pointis

    Corresponding author
    1. INSERM U 895, Université Paris Descartes, Paris, France
    2. Université de Nice Sophia Antipolis, Nice, France
    • INSERM U 895, C3M, 151 route Saint-Antoine de Ginestière BP 2 3194, 06204 Nice cedex 3, France
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Abstract

Follicle-stimulating hormone (FSH) is required for initiation and maintenance of spermatogenesis, a dynamic process of cell proliferation and maturation. By using FSH-gold particles and pulse-chase experiments, we analyzed the kinetics of FSH endocytosis in Sertoli and germ cells during development. Ultrastructural time-dependent analysis demonstrates that FSH was first located on plasma membrane, before being accumulated within the endosomal compartment, in the early endosomes, identified by morphological criteria and Rab-5 colocalization. Thereafter, FSH-gold was routed to the degradation pathway. The FSH endocytosis kinetic was similar in Sertoli cells, spermatogonia and spermatocytes. However, quantitative analysis of gold particles revealed differences in the dynamic of FSH accumulation in the endosomes between immature and mature rats. This age-dependent kinetic of FSH endocytosis, mostly detectable by ultrastructural analysis associated with quantitative data, argues for a potential new regulatory mechanism of the FSH signalling pathway that could occur during maturation of testicular cells. Developmental Dynamics 239:1113–1123, 2010. © 2010 Wiley-Liss, Inc.

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