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DVDY_22275_SuppFig-S1.tif9240KSupp. Fig. 1. Determining the probable location of grafted cells along the anteroposterior axis. A: Grafted embryos were divided into five regions consisting of the head (smaller cranial somites 1–4), anterior trunk (somites 5–9), mid trunk (somites 10–14), posterior trunk (somites 15–20), and tail (somites 21–45). For any given transplantation experiment, which typically consisted of 30 embryos, the specific regions containing labeled cells were noted. The frequency in which cells were found in each specific region was used as the numerator and the denominator consisted of the sum for all regions containing labeled cells within any given experiment. This fraction was then used to calculate the probability that grafted cells would give rise to myotome fibers in a specific region along the anteroposterior axis. B: The frequency in which labeled cells were observed in each region was calculated rather than the precise number of labeled cells that differentiated into myotome fibers, because the labeled cells were often positioned very close to one another making it challenging to discern the exact number of labeled cells. C: The percentages were then used to generate the data for each graph.

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