Patterns & Phenotypes
Dissecting the role of Fgf signaling during gastrulation and left-right axis formation in mouse embryos using chemical inhibitors
Version of Record online: 9 APR 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 6, pages 1768–1778, June 2010
How to Cite
Oki, S., Kitajima, K. and Meno, C. (2010), Dissecting the role of Fgf signaling during gastrulation and left-right axis formation in mouse embryos using chemical inhibitors. Dev. Dyn., 239: 1768–1778. doi: 10.1002/dvdy.22282
- Issue online: 17 MAY 2010
- Version of Record online: 9 APR 2010
- Manuscript Accepted: 22 FEB 2010
- MEXT. Grant Number: 21116002
- JSPS. Grant Number: 21657059
- Naito Foundation
- left-right axis;
- Fgf signaling
Fgf signaling plays pivotal roles in mouse gastrulation and left-right axis formation. However, although genetic analyses have revealed important aspects of Fgf signaling in these processes, the temporal resolution of genetic studies is low. Here, we combined whole-embryo culture with application of chemical compounds to inhibit Fgf signaling at specific time points. We found that sodium chlorate and PD173074 are potent inhibitors of Fgf signaling in early mouse embryos. Fgf signaling is required for the epithelial-to-mesenchymal transition of the primitive streak before the onset of gastrulation. Once gastrulation begins, Fgf signaling specifies mesodermal fates via the Ras/MAPK downstream cascade. Finally, Fgf signaling on the posterior side of the embryo during gastrulation induces Nodal expression in the node via Tbx6-Dll1, the initial event required for Nodal expression in the left lateral plate mesoderm. Developmental Dynamics 239:1768–1778, 2010. © 2010 Wiley-Liss, Inc.