Patterns & Phenotypes
Early development of medaka Oryzias latipes muscles as revealed by transgenic approaches using embryonic and larval types of myosin heavy chain genes
Article first published online: 20 APR 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 6, pages 1807–1817, June 2010
How to Cite
Ono, Y., Kinoshita, S., Ikeda, D. and Watabe, S. (2010), Early development of medaka Oryzias latipes muscles as revealed by transgenic approaches using embryonic and larval types of myosin heavy chain genes. Dev. Dyn., 239: 1807–1817. doi: 10.1002/dvdy.22298
- Issue published online: 17 MAY 2010
- Article first published online: 20 APR 2010
- Manuscript Accepted: 15 MAR 2010
- Japan Society for the Promotion of Science. Grant Numbers: 19108001, 18780160, 19-04576
Additional Supporting Information may be found in the online version of this article.
|DVDY_22298_sm_suppfig1.tif||3766K||Supporting Figure 1. A–C: A mononuclear muscle fiber expressing the emb1-1.9k-hrGFP transgene in the horizontal myoseptum of an embryo at 2 dpf (green). The nucleus in a mononuclear muscle fiber is indicated by arrowheads. A,C: Cell nuclei were stained by 4′, 6-diamidine-2′-phenylindole dihydrochloride (DAPI; magenta). Scale bars = 20 μm.|
|DVDY_22298_sm_suppfig2.tif||5034K||Supporting Figure 2. A–D: The expression of L1-2.6k-DsRed (A,B) and L2-4.0k-hrGFP (C,D) transgenes in zebrafish embryos. A and C were scanned in the inner part of trunk myotome and B and C in the superficial part of embryos at 1 days postfertilization (dpf). Both transgenes are expressed in the inner myotome (A,C), whereas only L2-4.0k-hrGFP is expressed in the superficial slow muscle labeled by F59 (D, arrowheads). Scale bars = 50 μm. Anterior is left in all panels.|
|Table_S1.xls||16K||Supporting Table 1. The effect of cyclopamine treatment on the survival rate of wild-type embryos at 2dpf|
|Table_S2.xls||15K||Supporting Table 2. Nucleotide sequence of primers for amplification of 5'-flanking region of mMYHs|
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