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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
DVDY_22301_sm_suppfig1.tif1441KSupporting Figure 1. A: In situ hybridization with a Gli2 riboprobe of embryonic day (E) 16.5 limbs reveals that Gli2 is highly expressed in distal chondrocytes and developing bone of wild-type (wt) limbs. B: The Gli2 transcript can be still detected in the nonhypertrophic chondrocytes of Ihh−/− mutants.
DVDY_22301_sm_suppfig2.tif3281KSupporting Figure 1. A–D: Bsp expression reveal differentiating osteoblasts in the perichondrium of the distal forearm adjacent to hypertrophic region in wild-type (wt) and Gli3−/−;Gli2-Col2cko (A,D, red arrows) but not in the perichondrium of Ihh−/−,Gli3−/− or Gli3−/−;Gli2-Prx1cko mutants (B,C, red arrows). E–H: Ossification can be observed in the corresponding region in wt and Gli3−/−;Gli2-Col2cko (E,H, blue arrows) but not in the perichondrium of Ihh−/−,Gli3−/− or Gli3−/−;Gli2-Prx1cko mutants (F,G, blue).

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