Patterns & Phenotypes
Identification of germ plasm-associated transcripts by microarray analysis of Xenopus vegetal cortex RNA
Article first published online: 20 APR 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 6, pages 1838–1848, June 2010
How to Cite
Cuykendall, T. N. and Houston, D. W. (2010), Identification of germ plasm-associated transcripts by microarray analysis of Xenopus vegetal cortex RNA. Dev. Dyn., 239: 1838–1848. doi: 10.1002/dvdy.22304
- Issue published online: 17 MAY 2010
- Article first published online: 20 APR 2010
- Manuscript Accepted: 22 MAR 2010
- NIH. Grant Number: 5R01 GM083999-02
- germ plasm;
- vegetal cortex;
- localized RNAs;
- primordial germ cells
RNA localization is a common mechanism for regulating cell structure and function. Localized RNAs in Xenopus oocytes are critical for early development, including germline specification by the germ plasm. Despite the importance of these localized RNAs, only approximately 25 have been identified and fewer are functionally characterized. Using microarrays, we identified a large set of localized RNAs from the vegetal cortex. Overall, our results indicate a minimum of 275 localized RNAs in oocytes, or 2–3% of maternal transcripts, which are in general agreement with previous findings. We further validated vegetal localization for 24 candidates and further characterized three genes expressed in the germ plasm. We identified novel germ plasm expression for reticulon 3.1, exd2 (a novel exonuclease-domain encoding gene), and a putative noncoding RNA. Further analysis of these and other localized RNAs will likely identify new functions of germ plasm and facilitate the identification of cis-acting RNA localization elements. Developmental Dynamics 239:1838–1848, 2010. © 2010 Wiley-Liss, Inc.