Prrxl1 is required for the generation of a subset of nociceptive glutamatergic superficial spinal dorsal horn neurons
Article first published online: 23 APR 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 6, pages 1684–1694, June 2010
How to Cite
Rebelo, S., Reguenga, C., Lopes, C. and Lima, D. (2010), Prrxl1 is required for the generation of a subset of nociceptive glutamatergic superficial spinal dorsal horn neurons. Dev. Dyn., 239: 1684–1694. doi: 10.1002/dvdy.22305
- Issue published online: 17 MAY 2010
- Article first published online: 23 APR 2010
- Manuscript Accepted: 25 MAR 2010
- FCT. Grant Number: POCI/SAU-NEU/59093/2004
Additional Supporting Information may be found in the online version of this article.
|DVDY_22305_sm_suppFig-S1.tif||59K||Supp. Fig. S1. Distribution of Prrxl1-, Tlx3-, and Lmx1b-expressing neurons in lamina I–III of the spinal superficial dorsal horn at P7. The three transcription factors are similarly distributed and the majority (around 60%( is located in lamina II.|
|DVDY_22305_sm_suppFig-S2.tif||90K||Supp. Fig. S2. Distribution of glutamatergic neuronal subpopulations defined by the combinatorial expression of Prrxl1, Tlx3, and Lmx1b in lamina I–III of the spinal superficial dorsal horn at P7. Prrxl1+/Tlx3−/Lmx1b− neurons are preferentially located in lamina II, while Prrxl1−/Tlx3+/Lmx1b+ neurons are concentrated in lamina III. The Prrxl1−/Tlx3+/Lmx1b+ subpopulation follows a distribution pattern similar to Prrxl1+/Tlx3+/Lmx1b+ neurons.|
|DVDY_22305_sm_suppFig-S3.tif||481K||Supp. Fig. S3. Prrxl1−/Tlx3+/Lmx1b+ subpopulation is not affected by the ablation of Prrxl1. Double-immunostaining for Tlx3 and Lmx1b showed that glutamatergic neurons present in the spinal dorsal horn of Prrxl1−/− mice express both Tlx3 and Lmx1b, representing the Prrxl1−/Tlx3+/Lmx1b+ subpopulation (see Fig. 3E). Scale bar = 50 μm.|
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