Patterns & Phenotypes
Expression patterns of the Wtx/Amer gene family during mouse embryonic development
Version of Record online: 12 MAY 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 6, pages 1867–1878, June 2010
How to Cite
Comai, G., Boutet, A., Neirijnck, Y. and Schedl, A. (2010), Expression patterns of the Wtx/Amer gene family during mouse embryonic development. Dev. Dyn., 239: 1867–1878. doi: 10.1002/dvdy.22313
- Issue online: 17 MAY 2010
- Version of Record online: 12 MAY 2010
- Manuscript Accepted: 30 MAR 2010
- Association pour la Recherche sur le Cancer (ARC). Grant Number: 1130
- Association for International Cancer Research (AICR). Grant Number: 09-0752
Additional Supporting Information may be found in the online version of this article.
|DVDY_22313_sm_SuppFig1.tif||5078K||Supporting Figure 1: Expression of Amer genes during postimplantation mouse embryonic development. A: Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of Amer genes in embryonic day (E) 7.5–E10.5 mouse embryos. Expression of the Hprt gene was used as a control of mRNA levels. The expression of the three Amer genes at E10.5 was used as a positive control for the assay. B: Whole-mount in situ hybridization analysis of Amer genes at E9.0. Wtx/Amer1 was diffusely expressed along the neuraxis, with a higher expression level in the anterior part (white arrowhead). This anterior-posterior gradient of expression was more evident for Amer2. Note the strong staining located in the anterior embryonic territories and prospective forebrain (black arrowhead). The dotted line indicates the approximate level of a gelatin section after whole-mount staining (lower inset). No Amer3 expression could be detected at this stage. C: Whole-mount in situ hybridization of Amer3 at E9.5 showing high levels of expression in the anterior part of the developing neural tube and cranial ganglia. The white asterisk is used to indicate trapping of stain within the neural tube. (a,b) Gelatin sections of the stained embryos at a level shown in the schematic drawing. White arrowheads are used to indicate regionalization of Amer3 expression in the neural epithelium. Fv, fourth ventricle, H, heart; Hb, hindbrain; Me, mesencephalon; Nt, neural tube; S, somites; Sc, spinal cord; Tc, telencephalon; VII/VIIIg, facio-acoustic ganglion; IXg, petrosal ganglion; Xg, nodose ganglion.|
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