Jarid2 is among a set of genes differentially regulated by Nkx2.5 during outflow tract morphogenesis
Article first published online: 14 JUN 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 7, pages 2024–2033, July 2010
How to Cite
Barth, J. L., Clark, C. D., Fresco, V. M., Knoll, E. P., Lee, B., Argraves, W. S. and Lee, K.-H. (2010), Jarid2 is among a set of genes differentially regulated by Nkx2.5 during outflow tract morphogenesis. Dev. Dyn., 239: 2024–2033. doi: 10.1002/dvdy.22341
- Issue published online: 14 JUN 2010
- Article first published online: 14 JUN 2010
- Manuscript Accepted: 15 MAY 2010
- NIH NCRR. Grant Numbers: P20 RR016434, HL095067
- congenital heart disease;
- secondary heart field;
- chromatin immunoprecipitation;
- double outlet right ventricle
Nkx2.5, a transcription factor implicated in human congenital heart disease, is required for regulation of second heart field (SHF) progenitors contributing to outflow tract (OFT). Here, we define a set of genes (Lrrn1, Elovl2, Safb, Slc39a6, Khdrbs1, Hoxb4, Fez1, Ccdc117, Jarid2, Nrcam, and Enpp3) expressed in SHF containing pharyngeal arch tissue whose regulation is dependent on Nkx2.5. Further investigation shows that Jarid2, which has been implicated in OFT morphogenesis, is a direct target of Nkx2.5 regulation. Jarid2 expression was up-regulated in SHF mesoderm of Nkx2.5-deficient embryos. Chromatin immunoprecipitation analysis showed Nkx2.5 interaction with consensus binding sites in the Jarid2 promoter in pharyngeal arch cells. Finally, Jarid2 promoter activity and mRNA expression levels were down-regulated by Nkx2.5 overexpression. Given the role of Jarid2 as a regulator of early cardiac proliferation, these findings highlight Jarid2 as one of several potential mediators of the critical role played by Nkx2.5 during OFT morphogenesis. Developmental Dynamics 239:2024–2033, 2010. © 2010 Wiley-Liss, Inc.