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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
DVDY_22367_sm_SuppFig1.tif10311KSupporting Information Figure 1. Cabin1 is expressed in the developing zebrafish nervous system. A–J: Embryos probed for Cabin1 sense control (B,D,F,H,J) contrast embryos probed with anti-sense Cabin1 (A,C,E,G,I) as determined by in situ hybridization (ISH). Lateral views of the head at 24–72 hours postfertilization (hpf; A–F) and coronal sections through stained embryos at 72 hpf (G–J). c, cerebellum; di, diencephalon; hb, hindbrain; m, midbrain; m/hb, midbrain/hindbrain boundary; oe, olfactory epithelium; pa, pharyngeal arches; po, preoptic area; r, retina; th, thalamus.
DVDY_22367_sm_SuppFig2.tif14442KSupporting Information Figure 2. Calcineurin subunits are expressed in unique temporal and spatial patterns. All calcineurin subunits are expressed in the nervous system during development as determined by whole embryo in situ hybridization (ISH). A–O: Lateral views of the head at 24 hpf (A,D,G,J,M), 48 hpf (B,E,H,K,N), and 72 hpf (C,F,I,L,O) and coronal sections of stained embryos at 72 hours postfertilization (hpf; C′–C′″; F′–F′″; I′–I′″; L′–L′″; O′–O′″). ac, anterior commissure; c, cerebellum; di, diencephalon; e, epiphysis; h, heart; hb, hindbrain; hg, hatching gland; hy, hypothalamus; l, liver; m, midbrain; ob, olfactory bulb; oe, olfactory epithelium; p, pallium; pa, pharyngeal arches; poc, post optic commissure; r, retina; s, skin; sp, subpallium; t, telencephalon; tec, tectum; teg, tegmentum. Some embryos have been manually deyolked.
DVDY_22367_sm_SuppFig3.tif14461KSupporting Information Figure 3. MEF2 isoforms are expressed in distinct temporal and spatial expression patterns in the developing CNS. All MEF2 isoforms are expressed in the nervous system during development as determined by whole embryo ISH. Lateral views of the head at 24 hpf (A, D, G, J, M), 48 hpf (B, E, H, K, N), and 72 hpf (C, F, I, L, O) and coronal sections of stained embryos at 48 hpf (I′–I′″) and 72 hpf (C′–C′″; F′–F′″; L′–L′″; O′–O′″). ac, anterior commissure; c, cerebellum; di, diencephalon; fb, finbud; h, heart; hb, hindbrain; m, midbrain, ob, olfactory bulb; oe, olfactory epithelium; p, pallium; pa, pharyngeal arches; png, peripheral nerve ganglia; s, skin; sp, subpallium; t, telencephalon; tec, tectum. Some embryos have been manually deyolked.
DVDY_22367_sm_SuppTables1to4.doc3903KSupporting Information Table 1. Accession numbers. Accession numbers from GenBank for nucleotide and protein sequences used in homology comparisons (Cabin1) or in situ hybridization (MEF2 and Calcineurin). Supporting Information Table 2. Sequences of ISH probes. Sequences were identified from GenBank submissions and verified by sequencing. Supporting Information Table 3. Summary of calcineurin gene expression. Relative expression is indicated by pluses: strong expression (+++), moderate expression (++), or weak expression (+). Supporting Information Table 4. Summary of MEF2 gene expression. Relative expression is indicated by pluses: strong expression (+++), moderate expression (++), or weak expression (+).

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