Patterns & Phenotypes
Identification of downstream targets of the Bone Morphogenetic Protein pathway in the Drosophila nervous system
Article first published online: 22 JUL 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 9, pages 2413–2425, September 2010
How to Cite
Kim, N. C. and Marqués, G. (2010), Identification of downstream targets of the Bone Morphogenetic Protein pathway in the Drosophila nervous system. Dev. Dyn., 239: 2413–2425. doi: 10.1002/dvdy.22368
- Issue published online: 24 AUG 2010
- Article first published online: 22 JUL 2010
- Manuscript Accepted: 16 JUN 2010
- Pew Scholars Program in the Biological Sciences. Grant Number: 3779sc
- wishful thinking;
- expression profiling
Bone Morphogenetic Protein (BMP) signaling mediated by the receptor Wishful thinking (Wit) is essential for nervous system development in Drosophila. Mutants lacking wit function show defects in neuromuscular junction development and function, specification of neurosecretory phenotypes, and eclosion behavior that result in lethality. The ligand is Glass bottom boat, the Drosophila ortholog of mammalian BMP-7, which acts as a retrograde signal through the Wit receptor. In order to identify transcriptional targets of the BMP pathway in the Drosophila nervous system, we have analyzed the gene expression profile of wit mutant larval central nervous system. Genes differentially expressed identified by microarray analysis have been verified by quantitative PCR and studied by in situ hybridization. Among the genes thus identified, we find solute transporters, neuropeptides, mitochondrial proteins, and novel genes. In addition, several genes are regulated by wit in an isoform-specific manner that suggest regulation of alternative splicing by BMP signaling. Developmental Dynamics 239:2413–2425, 2010. © 2010 Wiley-Liss, Inc.