Patterns & Phenotypes
Differential expression of cyclic nucleotide phosphodiesterases 4 in developing rat lung
Article first published online: 22 JUL 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 9, pages 2470–2478, September 2010
How to Cite
Lopez, E., Jarreau, P.-H., Zana, E., Franco-Montoya, M.-L., Schmitz, T., Evain-Brion, D., Bourbon, J., Delacourt, C. and Méhats, C. (2010), Differential expression of cyclic nucleotide phosphodiesterases 4 in developing rat lung. Dev. Dyn., 239: 2470–2478. doi: 10.1002/dvdy.22374
- Issue published online: 24 AUG 2010
- Article first published online: 22 JUL 2010
- Manuscript Accepted: 24 JUN 2010
- la Chancellerie des Universités de Paris. Grant Number: Legs Poix 2006
- Air Liquide Foundation
- lung development;
- phosphodiesterases 4;
- cyclic AMP
During the perinatal period, lungs undergo changes to adapt to air breathing. The genes involved in these changes are developmentally regulated by various signaling pathways, including the cyclic nucleotide cAMP. As PDE4s are critical enzymes for regulation of cAMP levels, the objective of this study was to investigate PDE4's ontogeny in developing rat lung during the perinatal period. Pulmonary PDE4 activity, PDE4A-D, PDE4B, and PDE4D variant expression levels, PDE4B and PDE4D protein levels, and PDE4D localization in distal lung were determined. PDE4 activity increased towards term, dropped at birth, and increased thereafter to reach a plateau at the end of the second week of life. PDE4B2 and PDE4D long forms demonstrated a pattern of expression that increased markedly at birth. After birth, PDE4D was expressed in alveolar epithelial and mesenchymal cells. The study, therefore, evidenced striking variations in expression patterns among the PDE4 family that differed from changes in global PDE4 activity. Developmental Dynamics 239:2470–2478, 2010. © 2010 Wiley-Liss, Inc.