Patterns & Phenotypes
The TGFβ type II receptor plays a critical role in the endothelial cells during cardiac development
Article first published online: 22 JUL 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 9, pages 2435–2442, September 2010
How to Cite
Robson, A., Allinson, K. R., Anderson, R. H., Henderson, D. J. and Arthur, H. M. (2010), The TGFβ type II receptor plays a critical role in the endothelial cells during cardiac development. Dev. Dyn., 239: 2435–2442. doi: 10.1002/dvdy.22376
- Issue published online: 24 AUG 2010
- Article first published online: 22 JUL 2010
- Manuscript Accepted: 22 JUN 2010
- Borwick Trust
- embryonic development;
- ventricular septal defect
TGFβ signalling is required for normal cardiac development. To investigate which cell types are involved, we used mice carrying a floxed Type II TGFβ receptor (Tgfbr2fl) allele and Cre-lox genetics to deplete this receptor in different regions of the heart. The three target tissues and corresponding Cre transgenic lines were atrioventricular myocardium (using cGata6-Cre), ventricular myocardium (using Mlc2v-Cre), and vascular endothelium (using tamoxifen-activated Cdh5(PAC)-CreERT2). Spatio-temporal Cre activity in each case was tracked via lacZ activation from the Rosa26R locus. Atrioventricular-myocardial-specific Tgfbr2 knockout (KO) embryos had short septal leaflets of the tricuspid valve, whereas ventricular myocardial-specific KO embryos mainly exhibited a normal cardiac phenotype. Inactivation of Tgfbr2 in endothelial cells from E11.5 resulted in deficient ventricular septation, accompanied by haemorrhage from cerebral blood vessels. We conclude that TGFβ signalling through the Tgfbr2 receptor, in endothelial cells, plays an important role in cardiac development, and is essential for cerebral vascular integrity. Developmental Dynamics 239:2435–2442, 2010. © 2010 Wiley-Liss, Inc.