Patterns & Phenotypes
Gene expression in the efferent ducts, epididymis, and vas deferens during embryonic development of the mouse
Article first published online: 22 JUL 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 9, pages 2479–2491, September 2010
How to Cite
Snyder, E. M., Small, C. L., Bomgardner, D., Xu, B., Evanoff, R., Griswold, M. D. and Hinton, B. T. (2010), Gene expression in the efferent ducts, epididymis, and vas deferens during embryonic development of the mouse. Dev. Dyn., 239: 2479–2491. doi: 10.1002/dvdy.22378
- Issue published online: 24 AUG 2010
- Article first published online: 22 JUL 2010
- Manuscript Accepted: 24 JUN 2010
- NIH-NICHD. Grant Numbers: HD-10808, KO8 award HD42058, HD52035
- The Eunice Kennedy Shriver Institute for CHHD. Grant Number: SCCPIR program U54 28934
Additional Supporting Information may be found in the online version of this article.
|DVDY_22378_sm_SuppFig1.tif||5709K||Supporting Information Figure 1. Schematic of the developing reproductive tract from E14.5 to P1. Dotted lines represent boundaries between tissues. At E14.5, the efferent ducts, epididymis, and vas deferens are uncoiled and the Mullerian duct is still fully intact. By E16.5, the Mullerian duct has undergone significant degeneration and coiling has been initiated in the efferent ducts and proximal portion of the epididymis. The Mullerian duct is no longer observed by E18.5. Coiling continues in the efferent ducts, and moves distally in the epididymis from E18.5 to birth. After birth, coiling is essentially complete in both the efferent ducts and epididymis. Throughout development and into adulthood, coiling is not observed in the vas deferens. ED (efferent ducts), Epi (epididymis), VD (vas deferens), WD (Wolffian duct), and MD (Mullerian duct).|
|DVDY_22378_sm_SuppFig2.tif||3659K||Supporting Information Figure 2. Light image of E14.5 dissected reproductive tract. Dotted lines represent boundaries between tissues. ED (efferent ducts), Epi (epididymis), VD (vas deferens), and MD (Mullerian duct).|
|DVDY_22378_sm_SuppFig3.tif||1324K||Supporting Information Figure 3. Tissue and temporal expression profiles of Hox transcripts in the developing reproductive tract. A: Tissue-enriched Hox transcripts. B: Tissue-enriched and developmentally regulated Hox transcript. C: Developmentally regulated Hox transcripts. Error bars represent standard deviation. Normalized microarray value represents the average of multiple probes where applicable.|
|DVDY_22378_sm_SuppFig4.tif||1453K||Supporting Information Figure 4. Tissue and temporal expression profiles of ontology identified cell adhesion genes in the developing reproductive tract. A: Vas deferens enriched. B: Epididymis and efferent duct enriched. Error bars represent standard deviation. Microarray raw score represents the average of multiple probes where applicable.|
|DVDY_22378_sm_SuppFig5.tif||3852K||Supporting Information Figure 5. Membrane-associated POSTN in adult heart and P1 epididymis. A: Control tissue (adult heart; Kii et al., 2010). B: P1 epididymis. POSTN (red) overlaid with nuclear counter stain (DAPI, blue).|
|DVDY_22378_sm_SuppTable1.doc||121K||Supporting Information Table 1. Functional Annotation Clustering of Genes Identified by Comparing Epididymis and Vas Deferens Across Development.|
|DVDY_22378_sm_SuppTable2.doc||44K||Supporting Information Table 2. Functional Annotation Clustering of Genes Identified by Comparing Epididymis and Efferent Ducts Across Development.|
|DVDY_22378_sm_SuppTable3.doc||51K||Supporting Information Table 3. Ontology Identified Cell Adhesion Genes Passing Criteria for Developmental Regulation and Either Vas Deferens or Epididymis and Efferent Duct Enrichment.|
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