Patterns & Phenotypes
Cath6, a bHLH atonal family proneural gene, negatively regulates neuronal differentiation in the retina
Article first published online: 20 AUG 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 9, pages 2492–2500, September 2010
How to Cite
Kubo, F. and Nakagawa, S. (2010), Cath6, a bHLH atonal family proneural gene, negatively regulates neuronal differentiation in the retina. Dev. Dyn., 239: 2492–2500. doi: 10.1002/dvdy.22381
- Issue published online: 24 AUG 2010
- Article first published online: 20 AUG 2010
- Manuscript Accepted: 30 JUN 2010
- Grant-in-Aid for Scientific Research. Grant Number: 19570216
- atonal superfamily;
- stem/progenitor cell
Basic helix–loop–helix (bHLH) transcription factors play important roles in cell type specification and differentiation during the development of the nervous system. In this study, we identified a chicken homolog of Atonal 8/ath6 (Cath6) and examined its role in the developing retina. Unlike other Atonal-family proneural genes that induce neuronal differentiation, Cath6 was expressed in stem cell-like progenitor cells in the marginal region of the retina, and its overexpression inhibited neuronal differentiation. A Cath6 fused with a VP16 transactivation domain recapitulated the inhibitory effect of Cath6 on neuronal differentiation, indicating that Cath6 functions as a transcription activator. These results demonstrate that Cath6 constitutes a unique member of the Atonal-family of genes in that it acts as a negative regulator of neuronal differentiation. Developmental Dynamics 239:2492–2500, 2010. © 2010 Wiley-Liss, Inc.