Patterns & Phenotypes
Neuron-specific expression of atp6v0c2 in zebrafish CNS
Article first published online: 24 AUG 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 9, pages 2501–2508, September 2010
How to Cite
Chung, A.-Y., Kim, M.-J., Kim, D., Bang, S., Hwang, S. W., Lim, C. S., Lee, S., Park, H.-C. and Huh, T.-L. (2010), Neuron-specific expression of atp6v0c2 in zebrafish CNS. Dev. Dyn., 239: 2501–2508. doi: 10.1002/dvdy.22383
- Issue published online: 24 AUG 2010
- Article first published online: 24 AUG 2010
- Manuscript Accepted: 3 JUL 2010
- Korean Health Technology R&D Project, Ministry for Health, Welfare & Family Affairs (code A084909), Republic of Korea
Additional Supporting Information may be found in the online version of this article.
|DVDY_22383_sm_suppinfoFigS1.tif||2028K||Supp. Fig. 1. Knock-down of atp6v0c2 in zebrafish embryos. A: Schematic diagrams of atp6v0c2 genomic DNA and splice sites targeted by E2I2 MOs (atp6v0c2 MO). atp6v0c2 MOs block the excision of the intron between exon 2 and exon 3, generating abnormal atp6v0c2 mRNA in which exon 2 and exon 3 are separated by the second intron (I2). B: Confirmation of the efficiency of atp6v0c2 MOs by reverse transcriptase-PCR (RT-PCR) analysis. RT-PCR with RNA from control embryos amplifies a 677-bp segment of the transcript. However, RT-PCR using RNA from atp6v0c2 MO-injected embryos failed to generate a PCR product, because excision of the 2,169-bp intron (I2) between exon 2 and exon 3 did not occur. C: Confirmation of the efficiency of atp6v0c2 MOs by in situ hybridization with atp6v0c2 RNA probe. All panels show lateral views of wild-type and atp6v0c2 MO-injected embryos analyzed by atp6v0c2. Since atp6v0c2 MOs interfere with splicing between exon2 and exon3 and generate an mRNA split by intron2, whole-mount RNA in situ hybridization did not detect any atp6v0c2 expression in the embryos injected with atp6v0c2 MOs.|
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