Patterns & Phenotypes
Echinoid regulates tracheal morphology and fusion cell fate in Drosophila
Article first published online: 20 AUG 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 239, Issue 9, pages 2509–2519, September 2010
How to Cite
Laplante, C., Paul, S. M., Beitel, G. J. and Nilson, L. A. (2010), Echinoid regulates tracheal morphology and fusion cell fate in Drosophila. Dev. Dyn., 239: 2509–2519. doi: 10.1002/dvdy.22386
- Issue published online: 24 AUG 2010
- Article first published online: 20 AUG 2010
- Manuscript Accepted: 7 JUL 2010
- NIH Lung Biology Training Grant. Grant Number: 5 T32 HL076139-0
- NIH. Grant Number: R01 GM069540
- Canadian Institutes of Health Research. Grant Number: IG1-89072
- Natural Sciences and Engineering Research Council of Canada. Grant Number: RGPIN 238938-07
Additional Supporting Information may be found in the online version of this article.
|DVDY_22386_sm_suppfig1.tif||6584K||Supp. Fig. 1. Echinoid (Ed) expression profile. A,A′: Stage 10 wild-type embryo stained for dp-ERK to highlight the tracheal placode (A) and Ed (A′). Ed is expressed uniformly in the tracheal placode (region surrounded by a dotted line in A′). B–B″: Stage 12 wild-type embryo stained for Armadillo (Arm; B) and Ed (B′; merge in B″ (Ed, green and Arm, red). Arm and Ed are expressed in all the trachea cells before fusion. C,C′: Stage 15 wild-type embryo stained for Arm (C) and Ed (C′) false-colored to show intensity (see scale of intensity levels at top right). Unlike Arm, Ed expression is not increased in the fusion cells (arrows, compare intensity levels). D: Stage 15 edM/Z embryo stained for Arm and false colored to show intensity levels. Arm expression is enriched in the fusion cells of edM/Z embryo in similarly to WT (arrows, compare intensity levels between C and D).|
|DVDY_22386_sm_suppfig2.tif||4753K||Supp. Fig. 2. Path length vs. distance. Wild-type embryo stained with WGA displayed as three different rotational views of a three-dimensional rendering (bottom left diagram displays the orientation of the X, Y, and Z axes). The path length described by the dorsal trunk between the transverse connectives of tracheal segments four to six (Path4-6) was measured by tracing a line through the tube following the three-dimensional aspects of the dorsal trunk (red line). The distance between the transverse connectives of tracheal segments four to six (Dist4-6) was measured by tracing a straight line between the start and finish point of the path (blue line).|
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