Patterns & Phenotypes
Three-dimensional localisation of NANOG, OCT4, and E-cadherin in porcine pre- and peri-implantation embryos
Article first published online: 18 NOV 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 240, Issue 1, pages 204–210, January 2011
How to Cite
Wolf, X. A., Serup, P. and Hyttel, P. (2011), Three-dimensional localisation of NANOG, OCT4, and E-cadherin in porcine pre- and peri-implantation embryos. Dev. Dyn., 240: 204–210. doi: 10.1002/dvdy.22491
- Issue published online: 22 DEC 2010
- Article first published online: 18 NOV 2010
- Manuscript Accepted: 18 OCT 2010
- Danish Agency for Science, Technology and Innovation
- Danish National Advanced Technology Foundation
- EU. Grant Numbers: EU FP7 Stem Cell Project “PartnErS” (218205;204, 523), EU FP7 Stem Cell Project “PluriSys” (223485)
- porcine pre-;
- peri-implantation embryos
The expression patterns of NANOG and OCT4 have previously been reported to differ markedly between mammalian species indicating distinct species-specific roles during development. We investigate the three-dimensional expression pattern of NANOG and OCT4 in porcine pre- and peri-implantation embryos. The expression of NANOG differed remarkably from that reported in other species. NANOG was not detected in the inner cell mass of hatched porcine blastocysts, but later appeared in the epiblast and hypoblast of spherical blastocysts where Rauber's layer had disintegrated. In pre-gastrulating, filamentous embryos NANOG was localised to nuclei in a minor portion of the epiblast cells in which E-CADHERIN seemed to be up-regulated and OCT4 down-regulated. Later NANOG was restricted to the potential PGCs. OCT4 was detected in inner cell mass, epiblast, and mesoderm, and we found that OCT4 expression, in contrast to earlier speculations, at least in hatched blastocysts, resembles the expression pattern in the mouse embryo. Developmental Dynamics, 2011. © 2010 Wiley-Liss, Inc.