Generation of a non-leaky heat shock–inducible Cre line for conditional Cre/lox strategies in zebrafish
Article first published online: 29 NOV 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 240, Issue 1, pages 108–115, January 2011
How to Cite
Hans, S., Freudenreich, D., Geffarth, M., Kaslin, J., Machate, A. and Brand, M. (2011), Generation of a non-leaky heat shock–inducible Cre line for conditional Cre/lox strategies in zebrafish. Dev. Dyn., 240: 108–115. doi: 10.1002/dvdy.22497
- Issue published online: 22 DEC 2010
- Article first published online: 29 NOV 2010
- Manuscript Accepted: 25 OCT 2010
- Deutsche Forschungsgemeinschaft. Grant Number: SFB655
- TU Dresden, European Union (Zf Health)
Additional Supporting Information may be found in the online version of this article.
|DVDY_22497_sm_SuppFigS1.tif||11779K||Supp. Fig. S1. Cre-mediated recombination of the (hsp70l:mCherry-T2A-CreERT2)#12 allele in the red-to-green reporter line in the absence and presence of heat and TAM. A: Scheme of the transgene combination of the embryos depicted in B–E. B: In the absence of heat and TAM, the combination of the Tg(hsp70l:mCherry-T2A-CreERT2)#12 allele with the red-to-green reporter line results in strong DsRed2 but no EGFP expression in double transgenic embryos. C: Application of TAM leads to mosaic EGFP expression in double transgenic embryos at permissive temperatures. D: In the absence of TAM, mosaic EGFP expression can be detected after heat treatment. E: Application of TAM and heat results in strong ubiquitous EGFP expression in double transgenic embryos. B–E: Lateral views of live 24-hpf embryos.|
|DVDY_22497_sm_SuppFigS2.tif||5443K||Supp. Fig. S2. Recombination efficiency of the (hsp70l:mCherry-T2A-CreERT2)#12. A: Scheme of the transgene combination and the dissection plane of the embryos depicted in B–D. Cross-sections of double transgenic embryos examined by immunohistochemistry against EGFP showing uniform EGFP expression in the retina (B), diencephalon (C), and hindbrain (D).|
|DVDY_22497_sm_SuppFigS3.tif||14813K||Supp. Fig. S3. Recombination of the (hsp70l:mCherry-T2A-CreERT2)#12 allele at later stages. A: Scheme of the transgene combination of the embryos depicted in C–E. B: Expression of DsRed2 in the red-to-green reporter line at 24, 48, and 72 hpf revealed by in situ hybridization. Fluorescent EGFP expression profile in double transgenic embryos after heat induction and TAM treatment at mid-gastrulation stages (C), 24 hpf (D), and 48 hpf (E), respectively.|
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