Hui Wang and Aline Bonnet contributed equally to this work.
Special Issue Techniques
Stable, conditional, and muscle-fiber-specific expression of electroporated transgenes in chick limb muscle cells
Article first published online: 2 DEC 2010
Copyright © 2010 Wiley-Liss, Inc.
Special Issue: Special Issue on Limb Development
Volume 240, Issue 5, pages 1223–1232, May 2011
How to Cite
Wang, H., Bonnet, A., Delfini, M. C., Kawakami, K., Takahashi, Y. and Duprez, D. (2011), Stable, conditional, and muscle-fiber-specific expression of electroporated transgenes in chick limb muscle cells. Dev. Dyn., 240: 1223–1232. doi: 10.1002/dvdy.22498
- Issue published online: 20 APR 2011
- Article first published online: 2 DEC 2010
- Manuscript Accepted: 21 OCT 2010
- ANR, FRM, AFM, NOE MYORES (6th PCRDT), University Paris 6, CNRS
Additional Supporting Information may be found in the online version of this article.
|DVDY_22498_sm_suppfig1.tif||5900K||Supporting Figure 1. A,B: Chick limb somites were electroporated at E2.5/HH15 with the inducible vectors (pCAGGS-T2TP, pT2K-CAGGS-rtTA-M2, and pT2K-BI-TRE-EGFP). In ovo GFP fluorescence was observed in chick embryos after E3.5 Dox injection, 6 hr (A) and 24 hr (B) after Dox injection. Arrowheads point to the GFP in somites, while the arrows point to the absence of GFP (A) and the presence of GFP (B) in limbs after Dox injection. The limbs are not at the same magnifications. C: Chick neural tubes were electroporated at E2/HH13 with inducible vectors (pCAGGS-T2TP, pT2K-CAGGS-rtTA-M2, and pT2K-BI-TRE-EGFP). In ovo GFP fluorescence was observed in chick neural tube, 5 hr after E3 Dox injection. White arrows point to the GFP in the neural tube.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.