An ATF2-based luciferase reporter to monitor non-canonical Wnt signaling in xenopus embryos
Article first published online: 2 DEC 2010
Copyright © 2010 Wiley-Liss, Inc.
Volume 240, Issue 1, pages 188–194, January 2011
How to Cite
Ohkawara, B. and Niehrs, C. (2011), An ATF2-based luciferase reporter to monitor non-canonical Wnt signaling in xenopus embryos. Dev. Dyn., 240: 188–194. doi: 10.1002/dvdy.22500
- Issue published online: 22 DEC 2010
- Article first published online: 2 DEC 2010
- Manuscript Accepted: 25 OCT 2010
- Wnt/PCP signaling;
Non-canonical/planar cell polarity (PCP) Wnt signaling plays important roles in embryonic development and tissue homeostasis, and is implicated in human disease. Monitoring Wnt/PCP signaling relies mostly on semi-quantitative bioassays or biochemical analysis. Here we describe a luciferase reporter assay based on an ATF2 response element, which faithfully monitors non-canonical Wnt signaling in Xenopus embryos. The assay is simple, quantitative, and robust. It can be used to detect non-canonical Wnt signaling changes following gain and loss of function of pathway components, including Wnt, Frizzled, Ror2, Disheveled, Rac1, MKK7, and JNK. Wnt/PCP signaling has recently been implicated in left-right asymmetry and our reporter assay suggests that in gastrula embryos there is a right-ward bias in Wnt/PCP signaling. We also mapped Wnt/PCP signaling in the early Xenopus embryo and find that it peaks in the dorso-vegetal region, paralleling Wnt/β-catenin signaling. Developmental Dynamics, 2011. © 2010 Wiley-Liss, Inc.